Enzymes

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31 Terms

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What is an enzyme?

A biological catalyst that speeds up chemical reactions without being used up.

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What type of molecule are enzymes?

Proteins.

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What does a catalyst do?

Increases the rate of a chemical reaction.

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What is the active site?

The part of the enzyme where the substrate binds.

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Why are enzymes specific?

Their active sites have a complementary shape to the substrate.

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What is a substrate?

The molecule an enzyme acts on.

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What is an enzyme–substrate complex?

A temporary structure formed when substrate binds to enzyme.

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Lock-and-key model

Substrate fits exactly into a rigid active site.

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Induced-fit model

Active site changes shape slightly when substrate binds.

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Which model is more accepted?

Induced-fit model.

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What is activation energy?

The minimum energy needed to start a reaction.

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How do enzymes lower activation energy?

By bringing substrates together and weakening bonds.

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Do enzymes change the overall energy of a reaction?

No.

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What is the optimum temperature for human enzymes?

About 37°C.

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What happens to enzymes at high temperatures?

They become denatured.

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What is denaturation?

Permanent change in enzyme shape causing loss of function.

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What is optimum pH?

The pH at which an enzyme works best.

18
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Optimum pH of amylase

About pH 7.

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Optimum pH of pepsin

About pH 2.

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Effect of increasing substrate concentration

Rate increases until enzymes are saturated.

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What is enzyme saturation?

All active sites are occupied.

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Effect of increasing enzyme concentration

Reaction rate increases if substrate is available.

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What is enzyme inhibition?

Reduction of enzyme activity.

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Competitive inhibition

Inhibitor competes with substrate for the active site.

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How to reduce competitive inhibition

Increase substrate concentration.

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Non-competitive inhibition

Inhibitor binds away from active site and changes enzyme shape.

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Can non-competitive inhibition be reversed?

No.

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What is a cofactor?

An inorganic ion needed for enzyme activity.

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Example of a cofactor

Magnesium ion (Mg²⁺).

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What is a coenzyme?

An organic molecule that helps enzyme action.

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Example of a coenzyme

NAD or FAD.