DIRECT ELISA TEST

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5 Terms

1
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Step 1

Antigens from patients sample are bound to inside of a well in a well plate

2
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Step 2

A detection antibody complementary to antigen is added

3
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Step 3

If antigen is present it will be immobilised on inside surface and antibody will bind to it

4
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Step 4

The well is washed to remove any unbound antibodies and substrate solution is added

5
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Step 5

If detection antibody is present there is a colour change- positive