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What characteristics do all cells share?
- plasma membranes
- chromosomes (DNA)
- cytoplasma
- Ribosomes (for protein synthesis)
What are the key points of the cell theory?
- All organisms are made of cells
- most basic unit of life
- All cells decend form earlier cells
How do eukaryotic and prokaryotic cells compare in size to each other?
Eukaryotic cells are larger than Prokaryotic cells
Organelle size
smaller and cells but larger than macromolecules
Biological macromolecules size
(like proteins/DNA) smaller than organelles
Viruses size
20-800 nm, smaller than prokaryotic cells
Prokaryotes make up which domain(s) of life?
Bacteria and Archaea
What structure(s) enclose prokaryotic cells ?
- plasma membrane
- cell wall (most)
capsule ( some for protein )
Eukaryotes make up which domain(s) of life?
Eukarya
Which eukaryotes have cell walls? Which do not? What are the cell walls made of?
plants: cellulose
fungi: Chitin
Animal cells: no cell wall
What is the function of the nucleus?
Stores DNA and controls the cells activity, like growth/ metabolism/ reproduction
What is chromatin?
DNA + proteins ( uncolied forming nucleus)
What is a chromosomes?
Condensed chromatin visible during cell division (2 complete sets of 23) ( total =46)
How do items get into and out of the nucleus?
through the nuclear pores that covered the surface of the nuclear envelope
What happens at the nucleolus? What materials are needed at the nucleolus to carry out the process ?
Function: make ribosomes
Needed: DNA( instructions) and proteins (from cytoplam)
What organelles/structures make up the endomembrane system ?
- nuclear envelope
- cell membrane
- Endoplasmic Reticulum
- Golgi apparatus
- Lysosomes
- Vesicles and Vacoles
What are the two types of ER ?
Smooth ER and Rough ER
How do we tell the difference between the two ERs, structurally?
Smooth ER: no Ribosomes, makes lipids, detoxification ( enzymes help)
Rough ER: has ribosomes, synthezies and folds proteins
What structure does a cell use to synthesize proteins?
Ribosomes
What are the possible destinations for proteins made on bond ribosomes
exported from the cell/ incerted in to membrane/ sent to the lysosome or Golgi
What does the Golgi apparatus look like?
a stack of pita bread
What happens in the Golgi apparatus?
proteins and lipids are modified, stored, then tagged with a "shipping" label before sent off.
How do things travel between the ER and Golgi?
transport vesicles
What are vacuoles?
large vesicles derived from the ER and Golgi apparatus
What are some different types ( and functions) of vacuoles?
Food Vacuoles: stores or digest food by, phagocytosis
Central Vacuoles: in plant cells, store water
Contractile Vacuoles: pump out: maintain solute concentration
What is the main function of a mitochondrion? What two compounds does this organelle need to carry out this
Function: Cellular respiration makes ATP
Need : Glucose and Oxygen
What are the structural parts of the mitochondria? What is the advantage of having additional cristae?
- Outer membrane
- inner membrane (folded into cristae)
- matrix (inside fluid)
advantage: increasing the surface area for ATP production
What is the main function of Chloroplast?
Function: photosynthesis ( makes sugar from sunlight)
What type of organelle is a chloroplast ?
energy- converting ( plasrid )
What are the structural parts of the Choloplasts?
Inner and Outer Membrane
flat sacs: thykoids stacked in grana: fluid around stacks is stroma
What types of Eukaryotes contain Chloroplasts
plants and algae
What is a peroxisome?
Break down fatty acids for energy/ break down toxins, detoxify hydrogen peroxide using catalse
What are the functions of the Eukaryotic cytoskeleton?
- mechanical support to maintain cell shape
- anachorage for organelles (move them)
- Cell mortality (division/movement)
What are the three components of the cytoskeleton ?
- micro tubules (thickest)
- micro filaments (thinnest)
- intermediate filament
What motor proteins are associated with each type of cytoskeletal fiber?
microbubules: dynein motor protein
microfilment: myosin motor proteins
Why do we need Cytoskeletal motor proteins?
to move vesicles, organelles, and the entire cell.
What are cillia?
Short, hair-like structures (move substance/ the cell)
What are flagella?
long, whip-like tail ( move the cell)
What are cilia and flagella used for?
movement
What cytoskeletal component makes up cilia and flagella?
Microtubules
How is the cytoskeletal structure within cilia and flagella?
Microtubules are arranged in the 9+2 pattern
What is a pseudopod?
a temporary extension of cytoplasm used for movement and feeding in some cells (like amobas)
What cytoskeletal component makes up a pseudopod?
microfilaments: they extend and retract to push the cell's membrane forward
What are the three types and functions of cell-cell junctions in animal cells?
tight: seals to prevent leakage (skin )
Gap: channels that allow small molecules to pass and communicate
Desmosomes: like rivets and fasten cells tightly together
Why don't we talk about cell-cell junctions in eukaryotes like yeast, Amoeba, Euglena, orParamecium?
because they are unicellular not multi celluar.
Cell Junctions are used to connect cells within the tissue of multicellular organisms
What is the main type of cell-cell junction in a plant cell? What is its function?
plasmodesmata: perforate the cell wall and allow passage of small molecules (similar to Gap Junctions and animal cells)
What components make up a membrane (like the plasma membrane) in a cell?
phospholipids : main structure
protein: transport and signaling
cholesterol: fluidity
carbohydrate: cell recognition
How are these components arranged in the membrane?
Arrangement : phospholipid bilayer with hydrophilic acids and hydrophobic sells inward
What is the fluid-mosaic model?
The membrane is the mosaic of protein molecules bobbing in a fluid bilayer of phospholipids.
What are some functions that proteins play in membranes?
Transport using ATP:
Enzyme activity:
Signal transduction:
Cell-to-cell recognition:
intercular joining.
Attachment to the cytoskeleton and extracellular matrix
What is the difference between integral and peripheral membrane proteins?
integral - span the membrane (proteins that extend entirely across the cell's lipid bilaye)
Peripheral - attached to the surface of the membrane
What is passive transport?
no energy is required in diffusion down is concentration
What is active transport?
requires energy ATP (high to low concentration)
What is diffusion?
tendency for molecules to spread out evenly into available space
What is osmosis?
diffusion of water across a selectively permeable membrane
What factors affect the rate of diffusion?
temperature, concentration gradient, surface areas, molecule size and distance
What happens to the movement of a solute (like glucose molecules or salt ions) when a cell is placed in a hypertonic solution? in a hypotonic solution? in an isotonic solution?
hypertonic solution: moves into cell
hypotonic solution: moves out of the cell
isotonic solution: no movements stay the same
What happens to the movement of water when a cell is placed in a hypertonic solution? in a hypotonic solution? in an isotonic solution?
hypertonic solution: water moves out (cell shrink)
hypotonic solution: water moves in (cell swells)
isotonic solution: no movement
What is crenation?
animal cell shrivels up in hypertonic solution
What is lysis?
animal cells burst in hypotonic solution
What is turgor pressure?
Pressure of water pushes against the cell wall in plants (Good)
What happens to turgor pressure when you place a plant cell in a hypertonic solution? a hypotonic solution? An isotonic solution? What is plasmolysis?
hypertonic solution: loses water, called plasmolysis (membrane pulls away from the wall
Hypotonic solution: gain water.. strong turgor pressure
isotonic solution: no pressure change.
What happens at equilibrium?
molecules still move but no net change in concentration across membrane
What is facilitated diffusion?
passive movement through channels /carrier proteins for molecules that cannot cross lipids bilayer easily
How do channel proteins differ from carrier proteins?
channel- proteins open pores
carrier proteins - change shape to move molecules
How do cells accomplish active transport?
using a transport protein (pump) poured by ATP
Why is active transport important?
Active transport allows molecules to move across cell membranes and epithelial membranes against a concentration gradient.
sodium-potassium pump
A transport protein in the plasma membrane of animal cells that actively transports sodium out of the cell and potassium into the cell. Moves 3 Na+ out and 2 K+ in.
What is exocytosis?
Fuses with plasma membrane & releases contents outside of the cell (exo = exit)
What is Endocytosis?
process by which the cell takes in materials that are too large to pass through (import)
What is Phagocytosis?
Cell eating
What is pinocytosis?
Cell drinking
What is receptor-mediated endocytosis?
specific molecules are taken in after they bind to a receptor