2.1 Cell Structure

What are the three types of microscopes?

• Light microscope

• Transmission electron microscope (TEMs)

• Scanning electron microscope (SEMs)

What observations can you make with light microscopes?

Structures within eukaryotic cells:

• nuclei

• sometimes mitochondria

• sometimes chloroplasts

What observations can you make with TEM microscopes?

Internal structures within cells & organelles e.g. ribosomes, endoplasmic reticulum or lysosomes

What observations can you make with SEM microscopes?

External, 3D structure of specimens

Why are stains used in slide preparation?

Stains make it easier for cells and cell structures to be seen

What is differential staining and why is it used?

A technique using two or more chemical stains to help distinguish between different organisms or organelles in a tissue sample

How do you calculate the magnification?

magnification = image size/actual size

What is magnification?

How many times bigger the image of a specimen observed is in compared to the actual size of the specimen

What is resolution?

The ability to distinguish between two separate points