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oligotrophic
low nutrient, specifically low in fixed N, P, and Fe
mixed culture
several species living together
environment in which most microbes live
biofilms
reversible attachment
microbe reacts with a surface - 1st step of biofilm formation
irreversible attachment
microbe attaches to surface via fimbriae or pili - 2nd step of biofilm formation
proliferation
multiple microbes clump together and build an extracellular matrix forms around them - 3rd step of biofilm formation
maturation
colonization by others, pieces begin to slough off - 4th step of biofilm formation
eutrophication
nutrient runoff causes the overgrowth and then death of aquatic algae
process that follows the initial algal bloom in eutrophication
the algae die/bust and release fixed carbon
process that follows algae death in eutrophication
a bloom of bacterial heterotrophs forms
process that follows bacterial heterotroph bloom in eutrophication
the bacteria use up the oxygen, and the system becomes anaerobic
process that follows the anaerobic environment in eutrophication
fish and other organisms die, the water begins to stink
organisms classified based on carbon source
autotrophs and heterotrophs
autotrophs
fix carbon from CO2 to organic carbon
heterotrophs
use organic carbon sources
organisms classified based on energy source
phototrophs, chemotrophs (organotrophs, lithotrophs)
phototrophs
use light energy
chemotrophs
use chemical energy
organotrophs
use electrons from organic compounds
lithotrophs
use electrons from minerals (nonorganic sources)
anaerobic respiration
O2 is NOT the terminal electron acceptor
oxidants
SO4, NO3, Fe3+, CO2
carbon fixation (carbon cycle)
CO2 → CH2O (reduction)
respiration (carbon cycle)
CH2O → CO2 (oxidation)
methane oxidation (carbon cycle)
CH4 → CO2 (oxidation)
methanogenesis (carbon cycle)
CO2 → CH4 (reduction)
nitrification (nitrogen cycle)
NH3 → NO2 → NO3 (oxidation)
ammonia oxidation (nitrogen cycle)
NH3 → NO2
nitrite oxidation (nitrogen cycle)
NO2 → NO3
denitrification (nitrogen cycle)
NO3 → N2O → N2 (reduction)
diazotrophs
nitrogen fixers
nitrogen fixation (nitrogen cycle)
N2 → NH3
assimilation (nitrogen cycle)
NH3 / NO3 → amino acids
sulfate reduction (sulfur cycle)
SO4 → H2S (dissimilatory)
assimilatory (sulfur cycle)
SO4 → protein
oxidation reaction (sulfur cycle)
H2S → S○ →SO4
vadose zone
aerobic area underground, reaching the water table
pump and treat
water is pumped into the ground (plume), and the dirty water that comes back out is treated at the surface
enhanced bioremediation
water, air, and nutrients are pumped into the ground, causing a redox reaction, and water and VOCs come out for treatment
in situ
treat in place
ex situ
dig it up and treat it elsewhere
composting
pile it up and let it degrade naturally
bioremediation technique that exhibits succession of species
composting