Dr. K- Use of Aseptic Technique

Based off her study guide

The most likely source of contamination is what?

operator involved in compounding

Microbial Contamination:

• source

• consequence

• source: microbes/ other pathogens

• consequence: infection

Chemical Contamination:

• source

• consequence

• source: chemicals and things that produce chemicals

• consequence: immunogenic and toxic effects

Physical Contamination:

• source

• consequence

• source: cores from stoppers, glass, particulate matter

• consequence: occlusions of vessels, thrombosis, phlebitis, death

General principles for LAFW are:

• Should operate _____________.

• All interior surfaces should be ___________ before use.

• Nothing should touch the ______________.

• Position away from __________________________.

• Should operate continuously.

• All interior surfaces should be cleaned before use.

• Nothing should touch the HEPA filter.

• Position away from excess traffic, doors, vents, fans.

General principles for LAFW are:

• Coughing/talking should be directed ______________________.

• Only objects ___________________________ should enter LAFW.

• Test every ___ months, when moved, or if damage suspected.

• Coughing/talking should be directed away from critical area.

• Only objects essential to compounding should enter LAFW.

• Test every 6 months, when moved, or if damage suspected.

People that are experiencing what should not enter the compounding CSP?

• rashes

• sunburns

• weeping sores

• respiratory infections

• conjunctivitis

Which of the following should not be worn into the compounding area:

SATA:

a. nail polish

b. fake nails

c. cosmetics

d. jewelry

e. coats

ALL SHOULD NOT BE WORN (a,b,c,d)

What is the order for donning PPE?

• dirtiest —> cleanest

• ante room —> buffer area

• Order can vary place to place but usually:

  • shoes→ hair→ face mask→ wash hands→ gown→ santize hands→ glove→ disinfect gloves

Where and when is handwashing performed?

• performed in ante area

• after jewelry and other objects removed

When washing your hands answer the following?

• What temp of water?

• Clean up to where?

• Clean under what?

• Water should run down __________ to ___________.

• Dry using what?

• warm water

• clean up to forearms

• clean under fingernails

• water should run down fingers to elbows

• dry using disposable lint free towel

What is the most common product used to wash hands?

chlorhexidine gluconate

Is IPA used for hand washing?

NO- it’s a disinfectant

Is Triclosan recommended for hand washing?

no

What are critical sites?

areas on container/devices with greatest risk of contamination

Critical Sites of a needle:

• hub

• tip

• shaft

• (anywhere but the cap)

Critical sites of a syringe:

• tip

• ribs of plunger

Critical sites of a vial:

• rubber closure

Critical sites of an ampule:

• neck/opening

Critical sites of a bag:

• additive/injection port

All critical sites must be exposed to the path of _________ from the HEPA filter.

1st air

Regarding air flow, the operator must be aware of what?

airflow DIRECTION

All aseptic manipulations should be done at least ___ inches within LAH.

6

How long should the hood be running before entering the LAH?

30 min

Before entering the LAH gloves should be disinfected with….

70% IPA

To disinfect a vial, use an alcohol wipe to wipe the rubber closure how many times?

3

To disinfect an ampule, wrap a wipe where?

neck of the ampule

After disinfecting the ampule with a wipe, use the wipe to do what?

break the ampule

To disinfect a bag, wipe the port how many times?

3

Since a vial is a closed system, what must be done to ensure that the correct volume is drawn up?

• draw up volume of air equal to amount of liquid being removed