Dr. K- Use of Aseptic Technique

Based off her study guide

The most likely source of contamination is what?

The most likely source of contamination is what?

operator involved in compounding

Microbial Contamination:

• source

• consequence

• source: microbes/ other pathogens

• consequence: infection

Chemical Contamination:

• source

• consequence

• source: chemicals and things that produce chemicals

• consequence: immunogenic and toxic effects

Physical Contamination:

• source

• consequence

• source: cores from stoppers, glass, particulate matter

• consequence: occlusions of vessels, thrombosis, phlebitis, death

General principles for LAFW are:

• Should operate _____________.

• All interior surfaces should be ___________ before use.

• Nothing should touch the ______________.

• Position away from __________________________.

• Should operate continuously.

• All interior surfaces should be cleaned before use.

• Nothing should touch the HEPA filter.

• Position away from excess traffic, doors, vents, fans.

General principles for LAFW are:

• Coughing/talking should be directed ______________________.

• Only objects ___________________________ should enter LAFW.

• Test every ___ months, when moved, or if damage suspected.

• Coughing/talking should be directed away from critical area.

• Only objects essential to compounding should enter LAFW.

• Test every 6 months, when moved, or if damage suspected.

People that are experiencing what should not enter the compounding CSP?

• rashes

• sunburns

• weeping sores

• respiratory infections

• conjunctivitis

Which of the following should not be worn into the compounding area:

SATA:

a. nail polish

b. fake nails

c. cosmetics

d. jewelry

e. coats

ALL SHOULD NOT BE WORN (a,b,c,d)

What is the order for donning PPE?

• dirtiest —> cleanest

• ante room —> buffer area

• Order can vary place to place but usually:

  • shoes→ hair→ face mask→ wash hands→ gown→ santize hands→ glove→ disinfect gloves

Where and when is handwashing performed?

• performed in ante area

• after jewelry and other objects removed

When washing your hands answer the following?

• What temp of water?

• Clean up to where?

• Clean under what?

• Water should run down __________ to ___________.

• Dry using what?

• warm water

• clean up to forearms

• clean under fingernails

• water should run down fingers to elbows

• dry using disposable lint free towel

What is the most common product used to wash hands?

chlorhexidine gluconate

Is IPA used for hand washing?

NO- it’s a disinfectant

Is Triclosan recommended for hand washing?

no

What are critical sites?

areas on container/devices with greatest risk of contamination

Critical Sites of a needle:

• hub

• tip

• shaft

• (anywhere but the cap)

Critical sites of a syringe:

• tip

• ribs of plunger

Critical sites of a vial:

• rubber closure

Critical sites of an ampule:

• neck/opening

Critical sites of a bag:

• additive/injection port

All critical sites must be exposed to the path of _________ from the HEPA filter.

1st air

Regarding air flow, the operator must be aware of what?

airflow DIRECTION

All aseptic manipulations should be done at least ___ inches within LAH.

6

How long should the hood be running before entering the LAH?

30 min

Before entering the LAH gloves should be disinfected with….

70% IPA

To disinfect a vial, use an alcohol wipe to wipe the rubber closure how many times?

3

To disinfect an ampule, wrap a wipe where?

neck of the ampule

After disinfecting the ampule with a wipe, use the wipe to do what?

break the ampule

To disinfect a bag, wipe the port how many times?

3

Since a vial is a closed system, what must be done to ensure that the correct volume is drawn up?

• draw up volume of air equal to amount of liquid being removed