Oxidase positive gram negative rods

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67 Terms

1
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Vibro cholerae is assoicated with?

  • fatal diarrhea and vomiting

  • pandemics

2
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Where are vibros typically found?

fresh, marine and brackish water

3
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What do vibrios typically cause?

Intestinal and extra intestinal disease

4
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How are vibro infections typically aquired?

Ingesting contaminated food (especially seafood) or water

5
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What are the two biogroups of vibrio cholera?

Classic and El tor

6
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What is the gram stain for vibrios?

Curved gram negative rods

7
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What are the catalase and oxidase reactions for vibrios?

Catalase and oxidase positive

8
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What is the second most common vibrio found in GI infections?

Vibrio parahaemolyticus

9
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What vibro causes disease in patients with chronic liver disease and is the second most serious infection?

Vibrio vulnificus

10
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Which vibrios are susceptible to 150 ug Vibriostatic agent?

All of them

11
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Which vibro is resistant to 10 ug of vibriostatic agent?

V. parahemolyticus

12
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What special media is used for vibrios?

Thiosulfate Citrate bile salt sucrose selective media (TCBS)

13
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Which vibros ferment sucrose?

V. cholera and alginolyticus (Yellow colonies on TCBS)

14
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Which vibros are sucrose non-fermentors?

V. vulnificus and parahaemolyticus (blue green colonies on TCBS)

15
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What is an enrichment media for isolating vibros?

Alkaline peptone water, high ph inhibits contaminants

16
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What is the LAO for vibrios?

+ / - / +

17
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Vibrios are glucose?

fermenters

18
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Which vibrio is VP positive?

Vibrio cholera and V. alginolyticus VP +

19
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Which vibrios are ONPG postivive?

V. cholerae and vulnificus

20
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Vibrios are motile, indole, gelatin and nitrate?

Positive for all

21
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Which vibrio is positive for lactose on MAC?

V. vulnificus, lactose fermenter

22
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How can vibrios and plesiomonas be differentiated?

Inositol fermentation, vibrios are negative

23
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What vibrio will grown in broth containing no NaCl but not in broth containing 6.5% NaCl?

Vibrio cholera, all vibrios require 1% NaCl to grow

24
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Which vibrio ferments salicin?

V. vulnificus

25
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What is the string test?

Mix vibrio colony with bile salt, lift colony with loop to see how long the string is to identify,

26
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What are the reactions for KSI and TSI for V. vulnificus?

A/A and A/A

27
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What are the reactions for KSI and TSI for V. parahaemolyticus?

AK/A and AK/A

28
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What are the reactions for KSI and TSI for V. chlorea?

AK/A and A/A

29
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Where is Aeromonas hydrophila found?

Ground water, reservoirs, lakes and rivers

30
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How is an aeromonas infection often acquired?

  • eating contaminated foods

  • drinking contaminated water

31
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Aeromonas hydrophila grows on BA as?

A beta-hemolytic colony

32
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Aeromonas hydrophlia on MAC is a?

Can be a LF or NLF

33
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What is Aeromonas hydrophila’s gram stain?

Straight gram negative rod

34
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What is aeromonas hydrophila’s reaction on KIA and TSI?

A/A or AK/A, A/A or AK/A

35
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Aeromonas is catalase and oxidase?

Positive

36
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Aeromonas is a glucose?

Fermenter

37
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Aeromonas is indole, VP and motile?

Positive for indole, VP and motility

38
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What is Aeromonas’s LAO reaction?

+/+/-

39
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Can aeromonas tolerate high NaCl?

Intolerant

40
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Aeromonas is —— to vibriostaic agent?

Resistant

41
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Aeromonas is inositol?

Negative

42
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Aeromonas is gelatin and esculin?

Positive

43
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What color colonies does aeromonas form on CIN?

Pink colonies

44
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What group was Plesiomonas shigelloides moved to?

Enterobacteriaceae

45
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Can Plesiomonas shigelloides tolerate salt and break down gelatin?

Intolerant to high salt and can not breakdown gelatin

46
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Plesiomonas grows as a?

Shiny, opaque, nonhemolytic colony

47
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What is plesiomonas’s gram stain?

Straight gram negative rod

48
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Plesiomonas ferments?

Lactose and inositol

49
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What is Plesiomona’s LAO?

+/+/+

50
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Plesiomonas is VP?

Negative

51
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Is plesiomonas susceptible to vibrostaic agent?

Yes

52
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What do most Campylobacters require for growth?

Microaerobic and capnophilic conditions (5-10% O2 to 10% CO2, 85% N2), use CAMY CVA media

53
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What is the gram stain of Camplyobacter?

Faintly staining Curved gram negative rods (S shapes, spirals, gull wings)

54
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What do camplyobacter colonies look like?

“runny” and spreading and are non-hemolytic

55
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How can you tell C. Jejuni and C. Coli appart?

Using hippurate hydrolysis, C. jejuni is positive while C. Coli in negative

56
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What temperature does C. jejuni grow optimally at?

42 degrees as moist colonies that spread along the streak

57
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What temperature do non-thermophilic campylobacters grow at?

37 degrees. Campylobacter fetus

58
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How long are Campy plates held before being reported out?

48-72 hours

59
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Campylobacter fetus is —- on KIA and TSI?

H2S positive

60
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Camplyobacter jejuni and coli are indoxyl acetate___ and susptible to?

Positive and naldixic acid

61
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How can you tell C. fetus and C. jejuni apart?

  • grows well at 25-35 degrees and not well at 42

  • Hippurate negative

  • Naldixic acid resistant

62
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What does Helicobacter pylori cause?

Type B gastritis which is associated with gastric cancer

63
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Can helicobacter pylori be cutured?

No but can be detected through gastric biopsy

64
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What is the gram stain of H. pylori?

Curved, gram negative rod

65
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H. pylori is catalse, oxidase and urease?

Positive for both and is a rapid urease producer.

66
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Campylobacters can also be isolated using?

A milipore filter.

67
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In the urea breath test what is being measured?

CO2, urea given with carbon isotopes(C13). H. pylori is a rapid urea utilizer so while convert the urea to CO2.