3.1-Brightfield-Microscopy

What is the total magnification if the ocular lens is 10x and the objective lens is 40x?

Total magnification is 400x (10x ocular x 40x objective).

What are the two types of light microscopes mentioned?

Compound light microscope and dissecting light microscope.

What does resolution in microscopy refer to?

Resolution is the ability of the lenses to distinguish between two points. makes image clearer

What is typical resolution for a microscope?

The maximum resolution is approximately 0.4 nm.

What is one key feature of a parfocal microscope?

The image remains in focus when changing from a lower-power objective lens to a higher-power lens.

What type of microscope uses a transmitted electron beam?

Transmission Electron Microscope (TEM).

What is a bright-field microscope?

A microscope that produces a dark image against a brighter background.

Where should the blue filter be placed?

The blue filter should be placed over the light source because it enhances the contrast allowing for a clearer image

How is immersion oil used in microscopy?

Immersion oil is placed between the slide and the 100x objective lens to reduce light refraction.

What is the function of the diaphragm in a microscope?

The diaphragm controls the amount of light entering the condenser.

What is the purpose of cleaning microscopes?

Cleaning is important to keep lenses free of contaminants which can impair image quality.

What is depth of field in microscopy?

the thickness of the specimen that is in focus at one time.

What are the primary parts of a compound light microscope?

Ocular lens, objective lenses, stage, condenser, diaphragm, illuminator, focusing knobs.

What is a scanning electron microscope (SEM) used for?

reflected electron beams

Define magnification

enlarges the object

define resolution

makes object clearer

what is the name of the 4x

scanning

what is the name of the 10x

low power

what is the name of the 40x

High dry

what is the name of the 100x

immergent oil

What is the Ocular lens

the eye pice of the microscope it has a 10x magnification