Micro Bio Lab 1

biosafety levels

practice, equipment, and designs put together to protect against organisms based on how hazardous it is, levels 1 to 4

BSL 1

organisms typically dont cause disease in healthy individuals

BSL 2

organisms are commonly encountered in the community and have environment and/or health hazard

BSL 3

organisms have local or exotic origin and are associated with respiratory transmission and serious or lethal diseases

BSL 4

organisms have a great potential for lethal infection

handwashing rules

wash hands when entering and exiting lab

Wescodyne rules

use one cap amount to wash lab bench when entering and exiting

glove rules

always wear gloves

ocular

10x view piece

nose piece

holds objectives

arm

holds the lens in place for viewing

objectives

scanning 4x, low power 10x, high dry 40x, oil immersion 100x

parfocal

when an object is in focus under one objective, it is in approximate focus under all objectives

stage

specimen held in place by stage clips, light passes through the bottom

stage controls

one knob moves stage up and down, the other moves it back and forth

coarse adjustment knob

knob raises and lowers the stage

fine adjustment knob

knob brings specimen into sharp focus

light source and power switch

turns on light, and shines to better view under microscope

light intensity knob

adjust amount of light going

condenser

concentrates light through the specimen by directing it into a sharp cone

iris diaphragm

part of condenser, adjusts the amount of light going through the specimen and increases contrast

resolution

clarity of an image

what do you do when you get oil on 40x lens

tell your lab ta

normal skin flora

organisms in relationship with host, difficult to remove by hand washing

transient skin flora

organisms that temporarily colonize the surface of the skin and are picked up, easy to remove by hand washing

how to label your agar plate

name, class time and day on the BOTTOM of the plate along with any plate content information