Amyloid Benhold Congo Red Stain Procedure

Flashcards reviewing the step-by-step manual procedure, solutions, timing, and microscopic visualization for the amyloid Benhold Congo red stain.

What is the primary purpose of the amyloid Benhold Congo red stain procedure?

It is used to identify extraneous protein deposits in amyloidosis.

What is amyloidosis?

A rare disorder that occurs when proteins gather in organs, affecting specific organs like the heart, kidneys, liver, and lungs, or spreading throughout the body.

What treatment options for amyloidosis are mentioned in the lecture?

Organ transplants, chemotherapy, or other targeted therapies.

What is the function of xylene in the initial preparation steps of the Benhold Congo red stain procedure?

Xylene is used for deparaffinization to clear off any extra paraffin (wax) from the tissue sample.

What is the purpose of using 100% and 95% alcohol in the procedure?

They are used as dehydrants to remove excess water present within the sample.

What is Solution A in the Benhold Congo red stain procedure, and what is its role?

Solution A is Congo red stain 1% aqueous, which stains any amyloids or proteins present in the tissue.

What is Solution B in the Benhold Congo red stain procedure, and what step does it carry out?

Solution B is alkaline alcohol, which serves as the differentiation step to clear the slide background.

What is Solution C in the Benhold Congo red stain procedure, and what is its function?

Solution C is the Mayer modified hematoxylin stain, which is used as the counterstain.

What is the exact deparaffinization step performed with xylene at the start of the procedure?

Three separate steps in fresh xylene containers for 3 minutes each.

How long is the tissue slide incubated in Solution A (Congo red stain 1% aqueous)?

1 hour (60 minutes).

How is the slide processed in Solution B (alkaline alcohol)?

It is submerged for 5 to 30 seconds while agitating constantly until the slide background is cleared.

How long is the slide kept in Solution C (Mayer modified hematoxylin stain)?

3 to 5 minutes, depending on the preference for nuclear stain intensity.

What color do amyloid tissue deposits appear as under non-polarized light with Congo red stain?

Red.

What visual feature is observed in amyloid-positive tissues under polarized light with Congo red stain?

Apple green birefringence.

Whose textbook was the colon biopsy specimen shown in the lecture taken from?

Freda Carson's textbook.