mol bio 2 - lecture 1 true

Gene X can be detected using PCR primers specific to Gene X.

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Molecular biology combines aspects of biochemistry and genetics.

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The study of gene structure and function is a key aspect of molecular biology.

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A gene is the basic unit of heredity.

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A gene encodes either an mRNA or a non-coding RNA molecule.

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Eukaryotic protein coding genes contain a transcribed region.

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The open reading frame (ORF) is part of the transcribed region of a gene.

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The 5′ untranslated region (5′ UTR) is found at the beginning of the mRNA transcript.

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The terminator is a regulatory element in eukaryotic protein coding genes.

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The sequence 'AAAAAA' represents a poly-A tail in mRNA.

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The 3′ untranslated region (3′ UTR) is located at the end of the mRNA transcript.

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Proteins and RNAs are primarily responsible for the functionality in the cell.

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Information in DNA is expressed in the form of proteins and RNAs.

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Gene expression refers to the generation of gene products.

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Proteins are generated through the translation of mRNA intermediates.

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Gene products are largely responsible for phenotypes/traits.

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Genes are expressed only when and where they are needed.

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Constitutive genes are always expressed without much regulation.

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Housekeeping genes are a type of constitutive gene.

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ACTB is a gene that encodes a protein component of the cytoskeleton.

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Facultative genes can be either inducible or repressible.

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The Lac Operon in E. coli is an example of facultative genes that are induced when lactose is present.

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HSPA8 is a gene that encodes a human heat shock protein and is part of the Hsp70 family.

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Regulated genes are another term for facultative genes.

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Gene expression can be regulated at multiple levels.

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Nucleic acid hybridization is one of the techniques for studying gene expression.

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Microarray is a technique for quantifying gene expression data.

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RNA seq is one of the techniques listed for studying gene expression.

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Cells and organisms can control gene expression at multiple levels.

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Post-translational regulation involves the control of protein activity after translation.

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The levels of regulating gene expression include chromatin and epigenomic/epigenetic factors.

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The course BIOL 3130 examines gene expression regulation.

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Some regulated genes can be either active or silent.

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The cell regulates how many transcripts are produced.

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Gene transcription can be regulated in a tissue specific manner.

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HBG1 encodes fetal specific ɣ globin.

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Gene transcription can be regulated in response to changes in the environment.

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Post transcriptional regulation occurs at the RNA level.

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Alternative splicing is a form of post transcriptional regulation.

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The steady state level of an RNA is influenced by its rates of synthesis and degradation.

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RNA localization refers to the transport of mRNAs to specific parts of the cell for expression.

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Post transcriptional regulation occurs at the RNA level.

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mRNA localization is an example of post transcriptional regulation.

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Post translational regulation occurs at the protein level.

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Proteolytic cleavages are an example of post translational modifications.

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Glycosylation, phosphorylation, and acetylation are examples of post translational modifications.

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Gene expression can be regulated at multiple levels.

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Nucleic acid hybridization is one of the techniques for studying gene expression.

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Microarray is a technique for quantifying and visualizing gene expression data.

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RNA seq is one of the techniques listed for studying gene expression.

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Genes are expressed through the processes of transcription and translation.

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Assays for detecting RNAs are used to determine if a gene is expressed.

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You can tell if a gene is expressed by using assays for detecting proteins.

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RNA fluorescence in situ hybridization (FISH) is one of the assays used to detect RNA transcripts.

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Microarray is a method that can tell you how abundant the RNA transcript is, relative to other transcripts.

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Reverse transcription PCR (RT PCR) is used to detect the presence of RNA transcripts.

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If a gene of interest is expressed in cultured cells or a tissue, the RNA transcript of that gene can be detected.

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Gene expression can be regulated at multiple levels.

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Nucleic acid hybridization is one of the techniques for studying gene expression.

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Microarray is a technique used for quantifying gene expression data.

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RNA seq is one of the techniques listed for detecting RNA.

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Hybridization is the process of annealing two complementary strands of nucleic acid from different sources.

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Two strands of nucleic acids anneal through base pairing because they have complementary sequences.

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DNA needs to be denatured prior to hybridization in order to separate the two strands.

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Nucleic Acid Hybridization involves denaturing RNA with secondary structure, melted DNA, and unfolded RNA.

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DNA can be denatured through heat or alkali conditions.

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Formaldehyde and urea are chemicals that can unfold RNA secondary structures.

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Excess probe is added under hybridizing conditions to detect nucleic acids by hybridization.

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Single stranded DNA or RNA can be used as a labeled probe in nucleic acid detection.

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True or False: Nucleic acid hybridization involves washing away unhybridized probes.

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True or False: The purpose of nucleic acid hybridization is to detect the location and intensity of the probe.

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Gene expression can be regulated at multiple levels.

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Nucleic acid hybridization is one of the techniques for studying gene expression.

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Microarray is a technique for quantifying gene expression data.

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RNA seq is one of the techniques listed for studying gene expression.

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RNA Fluorescence In Situ Hybridization (RNA FISH) allows visualization of specific RNA transcripts inside individual intact cells.

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RNA FISH can determine if a gene of interest is expressed in a cell.

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RNA FISH can show the location of transcripts within a cell, such as in the cytoplasm or nucleus.

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DAPI is a fluorescent stain that binds DNA.

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RNA Fluorescence In Situ Hybridization is abbreviated as RNA FISH.

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The text provides a link to a personal webpage.

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Gene expression can be regulated at multiple levels.

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Nucleic acid hybridization is one of the techniques for studying gene expression.

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Microarray is a technique for quantifying gene expression data.

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RNA seq is one of the techniques listed for studying gene expression.

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A northern blot can determine whether a specific RNA molecule is present in an RNA sample derived from cultured cells or tissues.

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If a gene is highly expressed, high levels of RNA transcripts for that gene can be detected in a northern blot.

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To perform a northern blot, the cells must be lysed to isolate all RNAs.

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Northern blotting is used to detect specific mRNAs in different tissues.

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GAPDH mRNA was isolated from rat tissues using a technique that involves size separation through electrophoresis.

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The purpose of the northern blot in this context is to determine whether a specific gene is expressed in different tissues.

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RNA is isolated from a culture of cells during sample preparation.

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The RNA samples are separated in an agarose gel by denaturing electrophoresis.

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Transfer of RNA from the agarose gel to the membrane occurs via capillary action.

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Molecular biology examines the structure and function of biomolecules.

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Gene expression can be regulated at multiple levels.

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Nucleic acid hybridization is one of the techniques for studying gene expression.

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Microarray is a technique used for quantifying gene expression data.

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RNA seq is one of the techniques listed for studying gene expression.

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The Northern blot method allows for the visualization of RNA bands on the membrane.

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