Video Notes: Aseptic Technique, Sterilization, and Biosafety (Page 1)

Question-and-answer flashcards covering key terms and concepts from aseptic technique, sterilization, disinfection, and biosafety levels as presented in Page 1 notes.

What is the definition of a colony?

single cell separated from other cells. Formed from cell aggregate to endospore

What does contamination mean?

presence of unwanted microorganisms

What does degerming mean?

doesn’t kill any microorganisms only washes them away with soap and water

What is disinfection (disinfect)?

kills most pathogenic bacteria and viruses by boiling, exposure to UV radiation, or chemicals like Bac-down

What does inoculate or inoculation mean?

addition of a specific microorganism to media on purpose

What is media?

nutrient containing substrate capable of allowing microorganisms to grow. - solid form: agar - liquid form: broth

What is sterilization?

to kill all living microorganisms, viruses, prions, and endospores achieved by autoclaving, incineration, ionizing radiation, and chemicals

What is turbidity?

cloudy appearance of the broth (media) meaning the increased number of cells

Identify the two aspects of aseptic techniques.

• prevention of contain action of media, surfaces, people and property - limit the spread of microorganisms to media, surfaces, people and property

What two conditions are used during the autoclave process?

pressure and temperature

What does the acronym BSL stand for?

Bio safety levels

Which BSL category includes the most dangerous microbes?

BSL-4

Which BSL category includes the least dangerous microbes?

BSL-1

What equipment is used to sterilize the inoculating loop and needle?

bunsen burner

What is the difference between sterilization and disinfection?

• Sterilization is the process that kills all living microorganisms, viruses, prions, and endospores; disinfection kills most pathogenic bacteria and viruses

Which level of control is better, sterilization or disinfection?

Sterilization

What are the four things destroyed by sterilization?

Microorganisms, viruses, prions, and endospores

What are the two things killed by disinfection?

bacteria and viruses