Plant Tissue Culture

Biotechnology

use biological agents, organisms, or systems to produce goods

explant

single cell, tissue, or organ

somatic cells

tissues that are vegetative and have complete genetic material

Germ cell

reproduction and half genetic material

embryo

rudimentary plant

propagule

tissuethat is divided for in vitro

plantlets

small complete plant via tissue culture

Axillary Shoot Culture

axillary branching, nodal culture, stool shoot

Aseptic seed culture

for orchids which have little food for seeds

embryo culture

removing an embryo
-ovary culture is the same

plantlet formation

-Axillary shoot formation
-adv shoot formation

axillary shoot formation/plantlet formation

-many lateral shoots grow rapid
1.axillary branching
2.nodal culture
3.stool shoots

meristem culture

obtain pathogen free
not for woody species

axillary shoot culture

involve stem cutting in vitro

axillary branching

many shoots from nodes provide a clump

nodal culture

single node on medium

stool shoots

several nodes on a shoot laid at an angle.Many shoots from axils

Axils?

apical domincance broken creates many shoots

adventitious shoot formation

direct- on explant without callus
indirect- callus on explant then shoots

advent. shoot factors

species, explant type, and hormones

Regenerate haploid plants

microspore or anther culture

callus formation

stationary callus, cell suspension, protoplast culture

stationary callus culture

subdivided every 4 weeks
high auxin and litte cytokinin

cell suspension culture

callus tissue in liquid culture/for studying

protoplast culture

without cell walls, removed by enzymes, studying

somatic embryogenesis

-refer to forming an underdeveloped plant
-somatic refers from veg cell. rather than zygote

somatic requirement

must go through all embryo stages

Seedling formation culture

embryo culture and aseptic seed culture

Callus formation culture

stationary callus culture
cell suspension culture
protoplast culture