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63 Terms

1
What are the three main phases of Cellular respiration?
Glycolysis, Krebs Cycle, Electron Transport Chain.
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2
What happens during Glycolysis?
A 6-carbon glucose molecule is broken down into two 3-carbon pyruvate molecules, producing 2 NADH and 2 net ATP.
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3
Where does the Krebs Cycle take place?
In the matrix of mitochondria.
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4
What is produced during the Krebs Cycle?
6 NADH, 2 FADH2, 4 CO2, and 2 ATP per glucose molecule.
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5
What is the main role of NADH and FADH2 in the electron transport chain?
They serve as electron carriers, donating electrons to the chain to create a proton gradient.
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6
What is chemiosmosis?
The process by which protons flow back through ATP synthase, leading to ATP synthesis.
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7
What is the theoretical maximum ATP yield from one glucose molecule during cellular respiration?
36 ATP.
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8
What is the difference between aerobic and anaerobic respiration?
Aerobic respiration requires oxygen and produces more ATP, while anaerobic respiration occurs without oxygen and generates less ATP through fermentation.
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9
What byproducts are produced during fermentation in yeast and muscle cells?
Ethanol in yeast and lactate in muscle cells.
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10
What do allosteric regulators do?
They can increase or decrease the activity of an enzyme.
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11
What are the central dogma processes of molecular biology?
DNA is transcribed to RNA, which is then translated into protein.
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12
What did Chargaff discover about DNA?
The amount of adenine equals thymine, and the amount of cytosine equals guanine.
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13
What did Meselson and Stahl demonstrate about DNA replication?
DNA replication is semiconservative.
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14
What is the difference between eukaryotic and prokaryotic transcription?
Eukaryotic transcription occurs in the nucleus and involves processing; prokaryotic occurs in the cytoplasm and can happen simultaneously with translation.
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15
What enzyme synthesizes RNA during transcription?
RNA polymerase.
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16
What is the role of the sigma factor in transcription?
It helps RNA polymerase bind to the promoter region.
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17
What is alternative splicing?
A process that allows for different combinations of exons to produce multiple proteins from a single gene.
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18
What is one function of the 5' cap on mRNA?
It stabilizes the mRNA and helps in ribosome recognition for translation.
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19
What does the poly-A tail do for mRNA?
It provides stability and aids in the termination of translation.
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20
What is the purpose of the Shine-Dalgarno sequence in prokaryotic mRNA?
It helps ribosomes recognize and bind to the mRNA.
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21
What is one key difference between the leading and lagging strands during DNA replication?
The leading strand is synthesized continuously, while the lagging strand is synthesized in short, discontinuous segments.
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22
What process breaks down ATP to release energy?
An exergonic process where ATP is converted to ADP and Pi.
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23
What enzyme removes RNA primers from DNA during replication?
DNA polymerase I.
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24
What is the significance of histones in eukaryotic DNA?
Histones are positively charged proteins that help package DNA into nucleosomes.
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25
What is telomerase and its function?
Telomerase extends unreplicated ends of DNA using its own internal RNA template.
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26
What do exonucleases do?
They hydrolyze nucleic acids from the ends of a DNA or RNA chain.
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27
How do intrinsic termination and Rho-dependent termination differ in prokaryotic transcription?
Intrinsic termination uses self-complementary sequences, while Rho-dependent termination involves the Rho protein pulling RNA off RNA polymerase.
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28
What is the process of splicing and its importance?
It removes introns and joins exons together, allowing for correct mRNA formation.
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29
What does the enzyme ligase do during DNA replication?
It seals nicks between adjacent nucleotides by forming phosphodiester bonds.
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30
What is the role of single-strand binding proteins (SSBPs) during DNA replication?
They hold apart separated strands of DNA to prevent them from reannealing.
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31
Which RNA polymerase transcribes mRNA in eukaryotic cells?
RNA polymerase II.
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32
What is included in a bacterial ribosome?
70S, which is made of 50S and 30S subunits.
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33
What effect does a mutation in the promoter region have on gene expression?
It would prevent transcription factors from binding, leading to no gene expression.
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34
What happens during the termination of translation?
Release factors recognize stop codons, leading to the release of the polypeptide chain and the disassembly of the ribosome.
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35
What is the term used for multiple ribosomes translating a single mRNA?
Polysome or polyribosome.
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36
What are the roles of nucleases in DNA replication?
They hydrolyze phosphodiester linkages in nucleic acids.
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37
What is the significance of the 30 nm fiber in chromatin?
It's a more condensed form of chromatin that plays a role in chromosome structure during cell division.
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38
What is an example of negative regulation in metabolic pathways?
Feedback inhibition, such as ATP binding to phosphofructokinase to decrease glycolysis rate.
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39
What are the benefits of alternative splicing?
It increases protein diversity from a limited number of genes.
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40
How is genetic code characterized?
It is degenerate (redundant), unambiguous, and non-overlapping.
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41
What is the function of aminoacyl-tRNA synthetases?
They attach amino acids to their corresponding tRNA molecules.
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42

What happens during fermentation in muscle cells when oxygen is scarce?

Lactate is produced as a byproduct.

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43

What are the two types of fermentation?

Alcoholic fermentation and lactic acid fermentation.

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44

What is the function of ATP synthase?

It synthesizes ATP from ADP and inorganic phosphate (Pi) during chemiosmosis.

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45

What is the role of the promoter in gene expression?

It is a DNA sequence that initiates transcription by RNA polymerase.

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46

What is an operon?

A cluster of genes regulated together, common in prokaryotic cells.

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47

What is the role of transcription factors?

They help regulate the transcription of specific genes by binding to nearby DNA.

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48

What does the term 'semi-conservative replication' mean?

Each new DNA molecule consists of one old strand and one newly synthesized strand.

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49

What is the role of DNA polymerase in DNA replication?

DNA polymerase synthesizes new DNA strands by adding nucleotides complementary to the template strand.

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50

What are introns and exons?

Introns are non-coding regions of a gene, while exons are coding regions that are translated into proteins.

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51

What is the final product of gene expression?

A functional protein.

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52

How do cells regulate metabolic pathways?

Through feedback inhibition and allosteric regulation.

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53

What is the significance of the 5' UTR in mRNA?

It plays a role in the regulation of translation and the stability of the mRNA.

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54

Who is Frederick Griffith and what did he discover?

Frederick Griffith discovered the phenomenon of transformation in bacteria through his experiments with Streptococcus pneumoniae, showing that genetic material could be transferred from one bacterium to another.

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55

Who is James Watson and what was his contribution?

James Watson, along with Francis Crick, discovered the double helix structure of DNA, which was pivotal in understanding the molecular basis of heredity.

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56

What did Rosalind Franklin contribute to the understanding of DNA?

Rosalind Franklin's X-ray diffraction images of DNA were critical in revealing the helical structure of DNA, which contributed to the discovery made by Watson and Crick.

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57

What did Gregor Mendel discover?

Gregor Mendel is known as the father of modern genetics for his foundational work on inheritance patterns in pea plants, establishing the principles of segregation and independent assortment.

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58

Who were Meselson and Stahl and what did they demonstrate?

Meselson and Stahl demonstrated that DNA replication is semiconservative, meaning that each new DNA molecule consists of one old strand and one new strand.

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59

What did Chargaff discover about DNA?

Chargaff discovered that in DNA, the amount of adenine equals thymine and the amount of cytosine equals guanine, a principle known as Chargaff's rules.

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60

What was the contribution of Barbara McClintock to genetics?

Barbara McClintock discovered transposable elements, or 'jumping genes,' which can move within and between chromosomes, challenging the notion of fixed genes.

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61

What did Elie Metchnikoff discover?

Elie Metchnikoff is known for his discovery of phagocytosis and the role of white blood cells in the immune response.

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62

Who is Paul Berg and what was his significance?

Paul Berg created the first recombinant DNA molecules, which laid the groundwork for genetic engineering.

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63

What was the discovery made by Sanger and his colleagues?

Frederick Sanger and his colleagues developed methods for sequencing DNA, including the Sanger sequencing technique, which became a fundamental method in molecular biology.

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