Histology Laboratory Assessment 1

digital pad of dog; h&e

what is the specimen shown and staining technique used

longitudinal section of lumbosacral nerve of dog; osmic acid

what is the specimen shown and staining technique used

duodenum of dog (mucus-secreting goblet cells); periodic acid-schiff

what is the specimen shown and staining technique used

cervix of sheep (epithelial cells); alcian blue/periodic acid-schiff

what is the specimen shown and staining technique used

adrenal of horse (reticular fibers); gordon and sweet

what is the specimen shown and staining technique used

stellate cells in the cerebellum of cat; cajal’s uranium silver

what is the specimen shown and staining technique used

tongue of dog; masson’s trichrome

what is the specimen shown and staining technique used

kidney of dog; gomori’s trichrome

what is the specimen shown and staining technique used

transmission electronic microscopy of a fibroblast of sheep

what is the specimen shown and microscopy technique used

scanning electronic microscopy of a kidney of a dog

what is the specimen shown and microscopy technique used

1. nucleus

2. nuclear membrane

3. cisternae of RER

4. plasmalemma

5. mitochondria

6. fat droplet

7. collagen fibrils

identify the numbered parts of the specimen

1. renal tubule

2. intersitial connective tissue

3. free erythrocyte

identify the numbered parts of the specimen

uterus of a cat; h&e; shrinkage

what is the specimen shown, staining technique used, and the artifact observed in the specimen

cartilage of dog; dark precipitates

what is the specimen shown and the artifact observed in the specimen

aorta of pig; fold and wrinkles

what is the specimen shown and the artifact observed in the specimen

esophagus of horse; masson’s trichrome; knicks in the knife

what is the specimen shown, staining technique used, and the artifact observed in the specimen

cloacal bursa or bird; h&e; pinching of tissue

what is the specimen shown, staining technique used, and the artifact observed in the specimen

spleen of dog; h&e; pinching of tissue

what is the specimen shown, staining technique used, and the artifact observed in the specimen

wall of jejunum; simple squamous epithelium

what is the specimen and epithelial tissue shown

outer wall of the jejunum; simple squamous epithelium

what is the specimen and epithelial tissue shown

1. tunica serosa

2. tunica muscularis

identify the specific parts

muscle layer and serous membrane of small intestine:

1. mesothelium

2. tunica serosa

3. tunica muscularis (outer layer)

4. tunica muscularis (inner layer)

identify the specific parts

blood vessel in the tunica serous of small intestine

what is the specimen shown

1. small vein

2. endothelium

3. small artery

identify the specific parts

cross section of kidney; simple cuboidal epithelium

what is the specimen and epithelial tissue shown

1. cortex

2. medulla

identify the specific parts

inner medulla of the kidney with collecting ducts

identify the specific part

1. lumen of collecting ducts

2. simple cuboidal epithelium

1. what are the white spaces between the collecting ducts

2. what epithelial tissue is present

wall of the small intestine of mouse; simple columnar epithelium

what is the specimen and epithelial tissue shown

villi of small intestine

what is the specimen shown

1. striated border

2. basement membrane

1. what are the borders present in the apical surface of the epithelial cells

2. what do you call the membrane in the inner surface of the villi

striated border

made up of numerous microvilli on the apical surface of the epithelial cells. these microvilli increase the surface area for absorption by the epithelial cells

1. apical border

2. basal border

what are the borders present in the slide

cross section of a canine trachea; pseudostratified columnar ciliated epithelium

what is the specimen and epithelial tissue shown

wall of a canine trachea

1. membrane

2. cartilage

identify the specimen and its specific parts

mucous membrane of a canine trachea

1. epithelium

identify the specimen and its specific parts

1. cilia

2. goblet cells

3. basement membrane

identify the specific parts

goblet cells

secrete mucus which trap particulate matter in the inhaled air

ciliary movement

produce a current that moves mucus and trapped particulate matter toward the outside

1. non-lactating mammary gland of a bovine; stratified cuboidal epithelium

2. lactiferous duct

1. what is the specimen and epithelial tissue shown

2. what is the white area shown in the slide

wall of a lactiferous ducts of a non-lactating mammary gland of a bovine; stratified cuboidal epithelium

what is the specimen and epithelial tissue shown

1. lactiferous duct

2. basement membrane

1. identify the middle white part

2. what membrane surrounds that part

sagittal section of the canine lip; stratified squamous epithelium

what is the specimen and epithelial tissue shown

1. mucous membrane on the inside, lower layer of the lips

2. skin on the outside upper layer of the lips

identify the specific parts

keratinized stratified squamous epithelium

what type of stratified squamous epithelium lines the skin of the canine lip

non-keratinized stratified squamous epithelium

what type of stratified squamous epithelium lines the mucous membrane of the canine lip

at the edge of the lip

where can you find the gradual transition from keratinized to non-keratinized stratified squamous epithelium in a canine lip

mucous membrane of the canine lip; non-keratinized stratified squamous epithelium

what is the specimen and epithelial tissue shown

basement membrane

what is the wavy part shown in the slide

skin of the canine lip; keratinized stratified squamous epithelium

what is the specimen and epithelial tissue shown

skin of the canine lip; keratinized stratified squamous epithelium

what is the specimen and epithelial tissue shown

tunica mucosa of an esophagus of a feline; non-keratinized stratified squamous epithelium

what is the specimen and epithelial tissue shown

1. skin of a foot pad of a canine

2. highly keratinized stratified squamous epithelium

3. keratin layer (stratum corneum) composed of dead cells

1. what is the specimen shown

2. what is the epithelial tissue present

3. what layer is present in the middle part of the epithelium and what it is composed of

basal layer of the epithelium

where do mitoses for cell replacement occur in stratified squamous epithelium

protective function

with what function is stratified squamous epithelium associated

cross section of the urinary bladder; transitional epithelium

what is the specimen and epithelial tissue shown

mucous membrane of the urinary bladder; transitional epithelium

what is the specimen and epithelial tissue shown

urinary bladder; transitional epithelium

what is the specimen and epithelial tissue shown

skin side of canine lip

1. arrow - epidermis

2. bracket - dermis

3. circle - skin glands

what is the specimen shown and its specific parts

skin glands of the skin side of the canine lip

1. sebaceous gland

2. hair follicle

3. sweat gland

what is the specimen shown and identify the parts

1. left - sebaceous gland

2. right - sweat gland

identify the left and right part

sebaceous gland

which gland secrete by the holocrine method

sweat gland

which gland secrete by the apocrine method

mandibular salivary gland

what is the specimen shown

secretory acini of the mandibular salivary gland

what are the parts surrounded by the black dots

1. upper - mucous cells

2. lower - serous cells

3. right - mucoserous acini

what are the parts surrounded by the white dots

merocrine method of secretion

what method of secretion does mucous and serous secrete by

secretory acini of the pancreas

what is the specimen shown

1. serous types of secretion

2. merocrine method of secretion

the pancreatic acini produce 1. what type of secretion and 2. secrete through what method

teased loose connective tissue

1. upper - vein

2. lower - artery

what is the specimen shown and its parts

teased loose connective tissue

what is the specimen shown

teased loose connective tissue:

1. macrophage

2. fibroblast

3. collagen fiber

what is the specimen shown and its parts

fibroblast

• responsible for the synthesis of the extracellular matrix of connective tissue (fibers, ground substance)

• primarily responsible for the regenerative capacity of many tissues, ex. healing of surgical incisions

• main cell type involve in the repair when spaces left after injury to tissues, whose cells do not divide, are filled by connective tissue which forms a scar

fibroblast

identify the specimen shown

it is responsible for the synthesis of the extracellular matrix of connective tissue (fibers, ground substance)

what is the function of fibroblast

they are primarily responsible for the regenerative capacity of many tissues, ex. healing of surgical incisions. spaces left after injury to tissues whose cells do not divide are filled by connective tissue, which forms a scar. the main cell type involved in this repair is the fibroblast

what purpose do fibroblast serve in wound healing?

1. fibroblasts

2. mast cells

identify the parts of the specimen shown in the slide

1. fibroblasts

2. mast cells

identify the parts of the specimen shown in the slide

fibroblast

• have oval nuclei and pale cytoplasm

• nucleus may appear euchromatic (pale) or heterochromatic (dark)

mast cells

what is the specimen shown

mast cells

the cells contain dark basophilic granules which also stain metachromatically

the granules contain the chemical mediators of the inflammatory response:

• histamine

• heparin

• eosinophil chemotactic factor of anaphylaxis

what do mast cells granules contain

1. upper - small artery

2. lower - blood vessels

3. mast cells

identify the parts of the specimen shown, what are the cells present?

mast cells in the perivascular connective tissue

what is the specimen shown

1. left - blood vessel

2. arrows - mast cells

3. upper and lower right - adipose cells

identify the parts of the specimen shown

adipose cells

• large cells that appear empty and contain large lipid droplets which were extracted during tissue preparation leaving a large vacuole

loose areolar connective tissue of canine inguinal region; india ink

what is the specimen shown and staining technique used

1. upper - adipose cells

2. lower - connective tissue cells (mostly inflammatory)

identify the parts of the loose connective tissue

1. neutrophils

2. macrophages

identify the inflammatory cells present in the loose connective tissue

1. neutrophil

2. macrophages

identify the inflammatory cells present in the loose connective tissue

macrophages

have phagocytosed carbon particles (of india ink), thus, their cytoplasm are filled with black granules

neutrophils

• cells with lobated nuclei

• first to migrate into an inflammatory site

• also phagocytic

1. macrophafe

2. neutrophils

identify the inflammatory cells present in the loose connective tissue

lymphocytes

identify the inflammatory cells present in the loose connective tissue

lymphoid cells

• also appear in an inflammatory site but at a later time

• can be identified by a dark round nucleus, which may be indented, surrounded by a scary amount of cytoplasm

jejunum of canine

what is the specimen shown

core of the villi of the small intestine of a canine

what is the specimen shown

tunica mucosa of the small intestine of a canine

1. lamina propria

2. crypts of lieberkuhn

what is the specimen shown and its parts

1. upper arrows - eosinophils

2. lower arrows - plasma cells

3. brackets - lamina propria

4. left and right - crypts of lieberkuhn

identify the parts

1. upper - eosinophils

2. middle left - lymphocyte

3. middle right - plasma cells

4. lower - fibroblast

identify the parts