DNA cloning and Restriction Endonuclease 3-12

list the 7 major steps of eukaryotic transcription and processing in the nucleus

initiation, capping, splicing, polyadenylation, termination, splicing again (if needed), exit nucleus

what is cloning DNA according to Dr. Hope

manipulating DNA by changing a base, cutting a section, or adding a section

describe the process of using plasmids to put genes into bacteria

cut plasmid with endonuclease, cut DNA with restriction endonuclease, ligate the fragments to plasmid, leave bacteria to replicate with plasmid

What are the two kinds of protein we use to put genes into bacteria using plasmids

restriction endonucleases and DNA ligase

what do restriction endonuclease recognize in DNA

palindromes

How do you read an RE table

arrow tells you where cut is, star = methylation point

what is a sticky end

there is an overhang

what is a blunt end

direct cut down the middle

why do you need two different restriction endonucleases to do directional cloning

because there is a 50/50 change that the DNA fragment will go in backwards if you dont