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What do phototrophs use as their main energy source? (A. Glucose, B. Light, C. Lipids, D. Proteins)
B. Light
Where does photosynthesis occur in plant and algal cells? (A. Mitochondria, B. Chloroplasts, C. Golgi, D. Nucleus)
B. Chloroplasts
What pigment is responsible for capturing light energy during photosynthesis? (A. Hemoglobin, B. Chlorophyll, C. Melanin, D. Carotene)
B. Chlorophyll
Do animal cells contain chloroplasts? (A. Yes, B. No)
B. No
Photosynthesis converts sunlight into what usable forms of energy? (A. ATP and NADPH, B. Glucose only, C. Pyruvate, D. FADH2)
A. ATP and NADPH
Chloroplasts are found in which organisms? (A. Animals only, B. Plants and algae, C. Bacteria only, D. Fungi only)
B. Plants and algae
What is photophosphorylation? (A. Light reaction of photosynthesis, B. Dark reaction, C. Glycolysis, D. Fermentation)
A. Light reaction of photosynthesis
What does photophosphorylation require? (A. Oxygen, B. Light, C. Glucose, D. Pyruvate)
B. Light
What are the two main products of photophosphorylation? (A. ATP and NADPH, B. CO2 and water, C. FADH2 and glucose, D. Lactate and ethanol)
A. ATP and NADPH
Where does photophosphorylation occur? (A. Cytoplasm, B. Thylakoid membrane, C. Mitochondrial matrix, D. Ribosome)
B. Thylakoid membrane
What does photophosphorylation generate to power ATP production? (A. Neutrons, B. Proton gradient, C. Calcium pump, D. Sodium current)
B. Proton gradient
Photophosphorylation is similar to which process in cellular respiration? (A. Krebs cycle, B. Electron transport chain, C. Fermentation, D. Glycolysis)
B. Electron transport chain
What is the main purpose of the Calvin cycle? (A. Make ATP, B. Fix carbon into organic molecules, C. Break down glucose, D. Produce oxygen)
B. Fix carbon into organic molecules
What molecule is the main product of the Calvin cycle that eventually forms glucose? (A. Pyruvate, B. G3P, C. NADP+, D. FADH2)
B. G3P
What do the dark reactions use from the light reactions? (A. O2 and glucose, B. ATP and NADPH, C. Water and CO2, D. Lactate and ethanol)
B. ATP and NADPH
Does the Calvin cycle require light to occur? (A. Yes, B. No)
B. No
Why is the Calvin cycle called a “dark reaction”? (A. It must occur at night, B. It does not directly require light, C. It only happens underground, D. It stops when exposed to light)
B. It does not directly require light
How many turns of the Calvin cycle are needed to make one glucose molecule? (A. 1, B. 2, C. 4, D. 6)
D. 6
During the Calvin cycle, ATP is converted into what? (A. G3P, B. ADP, C. NADH, D. O2)
B. ADP
Which molecule is regenerated and returned to the light reactions after the Calvin cycle? (A. NADP+, B. Glucose, C. Pyruvate, D. FADH2)
A. NADP+
The Calvin cycle is part of which overall process? (A. Glycolysis, B. Photosynthesis, C. Fermentation, D. Protein synthesis)
B. Photosynthesis
What do enzymes do in a cell? (A. Destroy substrates, B. Slow reactions, C. Catalyze biochemical reactions, D. Store energy)
C. Catalyze biochemical reactions
What suffix do most enzyme names end with? (A. -ose, B. -ase, C. -ine, D. -ate)
B. -ase
Protease catalyzes the breakdown of what? (A. Lipids, B. Proteins, C. Carbohydrates, D. DNA)
B. Proteins
What does catalase break down? (A. Glucose, B. CO2, C. Hydrogen peroxide, D. ATP)
C. Hydrogen peroxide
Why is catalase important for cell survival? (A. It produces glucose, B. It prevents oxidative damage, C. It stores oxygen, D. It forms membranes)
B. It prevents oxidative damage
Lipase catalyzes the breakdown of: (A. DNA, B. Fats (lipids), C. Proteins, D. Starch)
B. Fats (lipids)
Why can enzymes be detected in lab tests? (A. They are consumed in reactions, B. They change color, C. They are not destroyed during reactions, D. They become radioactive)
C. They are not destroyed during reactions
Enzyme specificity means each enzyme: (A. Works on many substrates, B. Works on one specific substrate, C. Is inactive, D. Works only in darkness)
B. Works on one specific substrate
What do hydrolases do? (A. Join molecules, B. Break bonds with water, C. Move electrons, D. Rearrange atoms)
B. Break bonds with water
Which enzyme class rearranges a molecule into a different isomer? (A. Isomerases, B. Transferases, C. Lyases, D. Ligases)
A. Isomerases
Which enzyme forms covalent bonds between molecules? (A. Lyase, B. Ligase, C. Oxidoreductase, D. Hydrolase)
B. Ligase
Which enzyme breaks bonds without hydrolysis or oxidation? (A. Lyase, B. Hydrolase, C. Transferase, D. Isomerase)
A. Lyase
Which enzyme type catalyzes electron transfer reactions? (A. Oxidoreductases, B. Ligases, C. Hydrolases, D. Isomerases)
A. Oxidoreductases
Which enzyme transfers functional groups between molecules? (A. Transferases, B. Hydrolases, C. Lyases, D. Oxidoreductases)
A. Transferases
What can inhibition of a single enzyme lead to in microbes? (A. Faster growth, B. Functional equilibrium, C. Survival failure, D. Increased ATP production)
C. Survival failure
What is an enzyme’s active site? (A. The storage area, B. The region where the substrate binds, C. The membrane channel, D. The waste site)
B. The region where the substrate binds
What determines enzyme specificity? (A. Temperature, B. Shape and chemistry of the active site, C. Color of the enzyme, D. Size of the product)
B. Shape and chemistry of the active site
What happens after a substrate binds to an enzyme’s active site? (A. The enzyme is destroyed, B. The substrate is converted into product, C. DNA replication begins, D. ATP is released)
B. The substrate is converted into product
Can enzymes be used again after releasing the product? (A. No, B. Yes)
B. Yes
What is the molecule that binds to the enzyme’s active site called? (A. Product, B. Substrate, C. Cofactor, D. Catalyst)
B. Substrate
Why are enzyme reactions clinically important? (A. They glow under UV, B. They can be detected and used for diagnosis, C. They create toxins, D. They prevent bacterial growth)
B. They can be detected and used for diagnosis
Why are enzyme types helpful in microbiology? (A. They determine cell size, B. They help identify microbes based on their biochemical processes, C. They control cell movement, D. They increase temperature)
B. They help identify microbes based on their biochemical processes