cell biology

What are the rules of making a scientific drawing?

-no arrow heads

-no overlapping lines

-no shading

-use a sharp pencil

-labels drawn with a ruler

-label must touch item

What are the four stains used in microscope work?

Methylene blue, iodine, acetic orcein and eosin

State what the stain methylene blue is used for

All perpouse stain

State what iodine stain is used for

For cellulose (yellow) and starch (blue/black)

State what acetic orecein is used for

To stain dna and chromosomes red

State what eosin stain is used for

To stain cytoplasm pink

Label the microscope

Define electron micrograph

Image produced by electron microscope

Define photo micrograph

Image produced by light microscope

Define magnification

Number of times enlarged

Define resolution

Clarity of details whilst being able to distinguish between two objects

Define differential staining

Staining technique to see cell structures

What is a stage micrometer/ graticule?

A glass slide with a measurable scale

Define organelle

Sub cellular structures that have roles within cells

What are the three kinds of microscope?

Light/optical, laser and electron.

What is the maximum magnification light/optical microscopes and electron microscopes?

X1500 vs X500,000

What kinds of specimen can each of the microscopes study?

Light: whole living specimens

Laser: whole living specimens

Electron: dehydrated and stained specimens

What causes poor resolution?

Light Wavelength is above 400nm, and cannot pass between objects.