HEMA 1 LEC: LEUKOPOEISIS (NEUTROPHILS) P2

-B2 microglobulin
-Collagenase
-Gelatinase
-Lysozyme
-Acetyltransferase

Tertiary granules contains?

-CD11b/CD18
-Alkaline phosphatase
-Vesicle-associated membrane-2
-CD10, CD13, CD14, CD16
-Cytochrome b558
-Complement 1q receptor
-Complement receptor-1

Secretory granules contains?

Chemical signals from damaged cells
leading to chemotaxis
↓
Margination (sticking to capillary
endothelium)
↓
Diapedesis
↓
Recognition of pathogen
↓
Attachment (Toll-like receptor of
phagocyte attaching to PAMPs)
↓
Ingestion
↓
Pathogen in phagosome
↓
Formation of phagolysosome
↓
Digestion & killing

Phagocytosis sequence

Nuclear & organelle membrane dissolves
↓
DNA release
↓
DNA + cytoplasmic enzymes
↓
Cell membrane ruptures
↓
NET release

Generation of NETs Sequence

-Myeloperoxidase
-Acid B-glycerophosphate
-Cathepsins
-Defensins
-Elastase
-Proteinase-3

Primary (Azurophilic) contains?

-B2 microglobulin
-Collagenase
-Gelatinase
-Lactoferrin
-Neutrophil gelatinase-associated lipocalin
-Transcobalamin I

Secondary granules contains?

7-30%

Normal values of WBCs in bone marrow?

50-70%

Normal values of WBCs in peripheral blood smear?

1.7-7.5 x10^9 /L

Normal values of Neutrophils Absolute value?

0.9-1 x10^9 cells/kg per day

Production of WBCs per day?

2.11 x10^9 cells/kg

Production of WBCs form mitotic pool?

5.6 x20^9 cells/kg

Production of WBCs form maturation pool?

-Circulating neutrophil pool (CNP)
-Marginated neutrophil pool (MNP)

In kinetics the peripheral blood, neutrophils are divided randomly into a?

Capillaries of the lungs

Majority of marginated neutrophil pool are in the?

6 days

Transit time

HSC to Myeloblast

4-6 days

Transit time

Myeloblast to maturation pool

6-8 hours

Neutrophil half-life in blood

14 days

Transit time

Blast stage to release mature granulocytes?

Primary - Secondary - Tertiary - Secretory

Granules type form order?

Primary - Secondary - Tertiary - Secretory

Granules type size (from largest to smallest)

Secretory - Tertiary - Secondary - Primary

Granules type degenerating order? (From First to Last)

Secretory - Tertiary - Secondary - Primary

First to Last to be released through exocytosis?

Primary (Azurophilic)

Present in immature cells in granulocyte series?

Primary (Azurophilic)

Has affinity to blue stains

Secondary granules

Contains antimicrobial agents?

Tertiary granules

Responsible for movement of neutrophils through connective tissue?

Secretory granules

Fuses to plasma membrane

Secretory granules

Responsible for carrying secondary granules to the surface of the cell for release of antimicrobial properties into circulation?

Neutrophil extracellular traps (NETs)

Extracellular threadlike structures believed to be represent chains of nucleosomes from DNA?

Neutrophil extracellular traps (NETs)

Have enzymes from neutrophil granules?

Neutrophil extracellular traps (NETs)

Have been shown to be able trap and kill gram + and gram - bacteria as fungi?

Transcobalamin I/R binder

Needed for Vitamin B12 absorption?

Oxygen dependent

Respiratory burst through the activation of NADPH oxidase.

NADPH oxidase

In oxygen dependent,
Respiratory burst through the activation of ___________

H2O2
Peroxidase

In oxygen dependent, ____and _____ are produced?

Oxygen independent

The pH within the phagosome becomes alkaline and then neutral, the pH at which digestive enzymes work?

Vitamin B12

Needed for nuclear maturation?

Neutrophil elastase

It targets the neutrophil nucleus?

CD11b/CD 18

From secretory granules that contribute to tight stationary binding between neutrophil and endothelial cells?

Gelatinase

Degrades denatured collagen, types IV, V collagen and activates chemokines (IL-8)?

IL-8

What chemokines did gelatinase activate?