Micro Lab Pure culture isolation

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7 Terms

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Culture

A population of microbes that is growing or has grown in a medium

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Inoculum

The bacteria that is introduced into a medium to initiate growth

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Culture

Nutrient material prepared to support the growth of microorganisms, transport or store microbes

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Liquid media

Broth

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Culture criteria

  • Culture medium must contain the nutrients that bacteria needs to grow

    • Energy source

    • Carbon, nitrogen, Sulfur, phosphorous

    • Vitamins, organic growth factors

  • Moisture

  • Proper osmotic balance

  • Proper pH

  • Suitable oxygen

  • Incubated at proper temp

  • Sterile

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Defined media

  • Precisely chemically defined

  • Exact chemical formula

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Complex media

  • Has an unknown

  • Extracts of animal plants, yeast

  • Rich in nutrients