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What is science?
systematic endeavor aimed at understanding the natural world through observation, experimentation, and logical reasoning
Inquiry
posing questions and seeking answers through systematic investigation
Observations: qualitative and quantitative
quantitative: describing qualities or characteristics
quantitative: involving numerical measurements
Reasoning: inductive and deductive
Inductive: involves making generalizations based on specific observations
deductive: logical inference to predict specific outcomes from general principles
hypothesis: definition and formulation
proposed explanation for a phenomenon or prediction that can be tested through experimentation.
how to write hypothesis
use If(cause), then (effect), because (rationale)
Null Hypothesis (H0)
states that there is no significant relationship between variables being studied
alternative hypothesis
proposes there is a significant relationship between variables studied
hypothesis
educated guess based on prior knowledge and observations, which can be tested through experiments
scientific law
describes an observable phenomenon that occurs consistently under specific conditions, often expressed mathematically
scientific theory
well-substantiated explanation supported by extensive evidence explaining the reasons behind a wide range of observations
Independent variable
the factor that is deliberately manipulated by the researcher
dependent variable
the factor that is measured or observed to assess the effect of the independent variable
positive control
the group is exposed to a treatment known to produce a positive result
negative control
this group is not to the treatment and should not produce a result (helps show external factors that influence outcome)
constants
all the factors that must be kept the same throughout the experiment for all groups
number of trials
having more trials per experiment is better to show how more robust and statistically significant the results will be
Statistics
Branch of mathematics that helps us understand and interpret data
Statistics in biology
used to summarize, describe, and analyze experimental data, helping scientists draw conclusions
Bell shaped curve
represents normal distributions, a common pattern where most data points are concentrated around the middle (mean) value, with fewer data points appearing as you move away from the mean.
Central Tendencies
measures that summarize a set of data by identifying the center point (Mean, Median, and Mode)
Mean
(Average) the sum of all data points divided by the number of data points.
Median
the middle value when the data points are ordered from least to greatest (central point of data)
Mode
most frequently occurring value in data set
variability
measures how spread out the data points are
range
difference between the highest and lowest values
standard deviation
shows how much the data points differ from the mean.
what does higher standard deviation mean
more spread out data (factors other than independent variable are causing the change)
low standard deviation
data points are closer to mean (means independent variable is causing changes)
Standard Error (SE)
measures how accurately the sample mean estimates the population mean (decreases as the sample size increases)
Error Bars
graphical representations of the variability of data on charts and graphs (represent range, standard deviation, or standard error)
Creating error bars
create normal bar graph, but use the mean value for each data set
add error bars above and below each bar to represent the standard error
Overlapping error bars
suggest that the difference between data sets may not be statistically significant
Non-overlapping error bars
suggest a significant difference between data sets
Chi-square (χ²)
a statistical test used to determine if there is a significant difference between observed and expected values in categorical data.
How to perform a chi-square test
state hypothesis
calculate expected frequencies
determine the expected frequencies based on hypothesis
calculate chi-square value
using the formula x²=sum((O-E)²/E)
O = number of observed frequencies
E= expected frequencies
determine degrees of freedom
degrees of freedom is the number of categories minus one
compare chi-square distribution
null hypothesis
there is no significant difference between the observed and expected frequencies
alternative hypothesis
there is significant difference between the observed and expected frequencies
matter
anything that takes up space and has mass
element
a pure substance that cannot be broken down into simpler substance by chemical means (made up of one kind of atom)
compound
a substance formed when two or more different elements combine in a fixed ratio
essential elements
elements that organisms need in large amounts to survive (CHON— make up of 96% of living matter)
trace elements
elements required by organisms in minute quantities (Fe, I, Zn)
Atomic number
number of protons in nucleus of atom
atomic mass
average mass of an element’s atoms, usually expressed in atomic mass units (amu)
groups
vertical columns that indicate elements with similar chemical properties
Periods
horizontal rows that indicate the number of electrons shells in the atoms of the elements
Bohr Model
represents electrons orbiting the nucleus in fixed energy levels or shells
Lewis Dot Model
Represents valence electrons as dots around the chemical symbol of an element
Octet Rule
Atoms tend to gain, lose, or share electrons to have full outer shell of 8 electrons, achieving stability similar to the noble gases
Electronegativity
A measure of an atom’s ability to attract and hold electrons in a chemical bond
High electronegativity = more attraction of electrons
Electroneg increases moving from left to right on the periodic table
Nonpolar Covalent Bond
electrons are shared equally between atoms
Polar covalent bonds
electrons are shared unequally leading to partial charges on atoms
Single bonds
one pair of electrons shared
Double bonds
two pairs of electrons shared
Triple bonds
Three bonds of electrons shared
Ionic bonds
Formed when one atom donates an electron to another atom, creating positively charged cations and negatively charged anions that attract eachother
Hydrogen bonds
Weak bonds between a hydrogen atom in one molecule and an electronegative atom in another molecule
Acids
substances that increase the concentration of hydrogen ions in a solution. They donate protons when dissolved in water
Bases
substances that reduce the concentration of hydrogen ions in a solution. They accept protons or produce hydroxide ions when dissolved in water
pH 7
neutral (pure water)
pH < 7
Acidic (more H+ ions)
pH > 7
Basic (more OH- ions)
Enzyme activity for pH
optimal pH range in which they function most efficiently, deviations form this range can denature enzymes, reducing their activity
enzymes
proteins that catalyze biochemical reactions
cellular processes with pH
cellular respiration and photosynthesis involve reactions that are pH-dependent
Homeostasis for pH
Organisms that maintain a stable internal pH through buffers, which are substances that minimize changes in pH by absorbing or releasing H+ or OH- ions
Buffers
crucial in biological systems to maintain a stable pH. They are mixtures of weak acids and their corresponding bases
Cohesion
the attraction between water molecules due to hydrogen bonding
Adhesion
the attraction between water molecules and other types of molecules
How do cohesion and adhesion work together in plants?
they facilitate transport from the roots to the leaves through the xylem in a process known as transpiration
What molecule is vital for all life forms and is polar (partial positive charge and partial negative charge)?
Water (H2O)
Capillary Action
ability of water to flow in narrow spaces without the assistance of external forces due to cohesion and adhesion
How does capillary action work in plants?
helps draw water up from the roots through tiny vessels in the replant stem and into leaves, brings nutrients and water from soil to rest of plant
High specific heat
Water can absorb or release large amounts of heat with only a slight change in its own temperature
Evaporative Cooling
When water evaporates, it takes heat away from the surface, cooling the organism. (eg. sweating)
Importance of density in water
the lower density of ice causes it to float on liquid water, insulating the water below and providing a habitat for aquatic life during cold seasons
Solvent
the substance that dissolves other substances to form a solution
Solute
the substance that is dissolved in a solvent
Solution
a homogeneous mixture of solvent and solute
Organic compounds
primarily composed carbon atoms bonded to hydrogen, oxygen, nitrogen, and other elements
Where are organic compounds found?
living organisms and include carbohydrates, lipids, proteins, and nucleic acids (Glucose, DNA, protein, and fats)
Inorganic Compounds
Inorganic Compounds do not primarily consist of carbon-hydrogen bonds. They include minerals, salts, and water
Where are inorganic compounds found?
non-living systems but are vital to life processes
Tetravalency in Carbon
Carbon has four valence electrons, allowing it to form four covalent bonds with other atoms, leading to a variety of complex molecules
Bonding capacity in Carbon
Carbon can form a single, double, and triple bonds, creating a range of molecular structures
Cantenation
Carbon can bond with other carbon atoms to form long chains and rings, essential for creating complex organic molecules
Hydrocarbon Chains
organic molecules consisting entirely of carbon and hydrogen
Types of hydrocarbon chains
single, double, or triple bonds
Importance of Carbon in Living systems