Enzyme Activity Flashcards
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Vocabulary flashcards for key terms related to enzyme activity measurement.
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14 Terms
Peroxidase
An enzyme that eliminates toxic H2O2 (hydrogen peroxide), found in nearly all living organisms.
H2O2 (Hydrogen Peroxide)
A common disinfectant and bleaching agent that is a byproduct of normal metabolic activity in cells and is removed by peroxidase.
-ase suffix
Signifies that a molecule is an enzyme.
Heme
A coenzyme molecule found in the active site of peroxidase, also found in hemoglobin; a porphyrin molecule with an iron ion at its center.
Substrates of Peroxidase
H2O2 and a phenolic compound (such as guaiacol), both must be present for the reaction to occur.
Guaiacol
A phenolic compound used as a substrate for peroxidase in turnip tissue, oxidized to a quinone during the reaction.
Quinone
The oxidized form of a phenolic compound (like guaiacol) in the peroxidase-catalyzed reaction. Reacts to form colored complexes.
Colorimeter
An instrument used to measure the amount of colored product formed in the peroxidase reaction, typically at 500 nm wavelength.
SpectroVis Plus
A colorimeter used to quantify the amount of product formed in the peroxidase-catalyzed reaction.
Assay
The process of combining specified volumes of various solutions in a test tube and then measuring a change in the %T with the colorimeter.
%Transmittance (%T)
A measure of how much light passes through a solution. Decreases as the concentration of colored product increases.
Independent Variable (in H2O2 experiment)
The H2O2 concentration in each assay, which will be varied to observe its effect on reaction rate.
Dependent Variable (in H2O2 experiment)
The reaction rate, calculated by dividing the amount of product formed (200 micromoles) by the time (in seconds) for the %T value to fall from 70%T to 50%T.
Michaelis-Menten Curve
The expected shape of the data plot of Reaction Rate (dependent variable) versus H2O2 Concentration (independent variable), showing saturation of the enzyme at high substrate concentrations.