BB ASCP

ascp immunoheme

washed rbcs

hx of severe allergic rxn

good for 24hrs after washing

washed with saline

rbcs leykocytes reduced

hx of febrile rxn

filtration or apheresis processing

rbcs irradiated

immunodeficiency

malignancy

bone marrow transplant

transfusion w/ family member’s blood

intrauterine and neonatal transfusions

for prevention of graft vs host disease

kills donor t cells

FFP

deficiency of coag factors

contains all coag factors

cryo

fibrinogen and factor 8 deficiencies

used for hemophilia A and VWF disease only if factor 8 concentrate or recombinant factor preparations not available

platelets

severe thrombocytopenia or abnormal plt function

one unit should increase plt count by 5-10k

apheresis plt

apheresis

severe thrombocytopenia

leukocyte-reduced platelets

wbs removed by filtration or during apheresis processing

recurrent febrile rxn and to decrease risk of CMV transmission or HLA alloimmunization

genotype for A

AA

AO

genotype for B

BB

BO

genotype for AB

AB

genotype for O

OO

frequency for type O in whites

45%

frequency for type O in blacks

49%

frequency for type O in Hispanics

57%

frequency for type O in Asians

40%

frequency for type A in whites

40

frequency for type A in blacks

27

frequency for type A in Hispanics

31

frequency for type A in Asians

27

frequency for type B in whites

11

frequency for type B in blacks

19

frequency for type B in Asians

25

frequency for type AB in whites

4

frequency for type AB in blacks

4

frequency for type AB in Hispanics

2

frequency for type AB in Asians

7

missing isoagglutinin group O discrepancies

incubate reverse grouping at RT for 30 mins

if still negative incubate at 4C for 15-30 mins

A2 with anti-A1

type RBCs with anti-A1

test serum with several additional A1, A2, and O cells

rouleaux

use washed RBCs suspended in saline for forward grouping

perform saline replacement technique in reverse grouping

AB with cold alloantibody

perform antibody panel at RT

repeat reverse grouping with A1 and B cells that lack corresponding antigen

A2B with anti-A1

type cells with anti-A1

acquired B antigen

check medical hx for infection by GI bacteria

retype RBCs w/ different monoclonal anti-B or acidified human anti-B

RH +

DD, Dd

RH=

dd

D antigen

Rh0

Rh1

C antigen

rh’

Rh2

E anitgen

rh”

Rh3

c antigen

hr’

Rh4

e antigen

hr”

Rh5

% for whites: D antigen

85

% for whites: C

68

% for whites: E

29

% for whites: c

80

% for whites: e

98

% for blacks: D

92

% for blacks: C

27

% for blacks: E

22

% for blacks: c

96

% for blacks: e

98

Rh0

Dce

Rh1

DCe

Rh2

DcE

Rhz

DCE

rh

dce

rh’

dCe

rh”

dcE

rhy

dCE

what’s the population of highest frequency of Rh1

Asians 70%

what’s the population of highest frequency of Rh0

blacks 44%

what’s the population of highest frequency of Rh2

Asians 21%

weak D

mommas =

baby =

lowest antigen of whites

Kell

lowest antigen of blacks

Kell 1st

fya/fyb (duffy)

clinically significant ab

ABO

Kell

Duffy

Kidd

SSu

cold ab

M
N
P

complement

helpful in investigating of immune hemolytic anemia

anti-C3d or anti-C3b

IgG

can be used for routine compatibility tests and ab ID

detects clinically significant abs

IgG and C3d

DAT

graft vs host disease

rash, nausea, vomiting, diarrhea, fever, pancytopenia, usually fatal

viable T lymphs in donor blood attack recipient

alloimmunization

no clinical signs unless subsequently exposed to same foreign antigens

development of abs to foreign RBCs/WBCs/PLTs and plasma proteins following transfusion