Generation of a Mutant Library

What screen is ideal for an unbiased search for longevity genes

Why use a forward genetics screen

the pathways are unknown

What kind of genes does a forward genetics screen search for

longevity genes

Methods to generate a mutant library

transposon mutagenesis, CRISPR/CAS9

What system does transposon mutagenesis use

piggybac or Hermes

What can using transposon mutagenesis create

a large scale library of random gene disruptions

What will strains where the transposon landed in an ageing gene show

altered survival

When can crispr be used

when a genome is sequenced

What can crispr be used to generate

a systemic collection of gene knock outs

What can crispr be used to introduce

premature stop codons efficiently across the geno