BB 13: Blood and Blood Components (Pt. 1)

volume variations and quantity of cellular elements are large for _____.

apheresis

WB must be collected within ____ minutes.

15-20

platelets are stored at _____°C.

20-24°C

plasma are stored at _____°C.

1-6°C

platelets and plasma components must be created within ____ hours from initial collection time.

8

CPD, CP2D, and ACD additives have a ____ day expiration.

21

CPDA-1 has a ____ day expiration.

35

additive solutions (e.g., AS-1, AS-3, AS-5) have a ____ day expiration.

42

it is against the law to use any blood/blood product past its expiration date. true or false?

true

it is against the law to use any blood/blood product that exceeds any of the applicable temperature ranges. true or false?

true

____ storage lesion biochemical changes involve:

• decreased glucose

• decreased ATP (causes increased Hb O2 affinity)

• decreased 2,3 DPG declines rapidly after 1-2 weeks (causes increased Hb O2 affinity)

• decreased pH

RBC

____ storage lesion biochemical changes involve:

• increased K+ in supernatant

• increased free Hb

RBC

____ storage lesion morphological changes involve:

• irreversible shape deformity

• increased osmotic fragility

RBC

____ storage lesion biochemical changes involve:

• decreased glucose

• decreased pH

• increased LDH

platelet

____ storage lesion morphological changes involve:

• very fragile/fragmentation; degranulation occurs

• without continuous rotation, PLTs will clump

• irreversible shape deformity from discoid to spherical

• decreased in-vivo recovery, survival, and function

platelet

____ storage lesion biochemical changes involve:

• deterioration of heat liable coagulation factors

  • e.g., factor V and VIII

plasma

____ storage lesion morphological changes involve:

• there are no cellular elements found in plasma products

  • there is no morphological changes occurring

plasma

the life span of RBCs in the body are ____ days.

120

the life span of platelets in the body are ____ days.

10

the life span of WBCs in the body are ____ days.

1-3

the ____ blood label is used on all blood products.

ISBT

____ indications include:

• acute hemorrhaging

  • trauma or massive bleed

• volume replacement

  • hypovolemia

• increase oxygen carrying capacity to vital organs

  • e.g., brain, liver, heart, kidneys, etc.

WB

the storage temperature for whole blood is ____°C.

1-6°C

the expiration of whole blood with ACD, CPD, or CP2D is ____ days.

21

the transport temperature of whole blood is _____°C.

1-10°C

the use of _____ as the resuscitation product of choice for the treatment of hemorrhagic shock for all casualties has been endorsed by the Joint Trauma System, the Defense Committee on Trauma, and the Armed Services Blood Program.

LTOWB

the storage temperature for LTOWB is ____°C.

1-6°C

the expiration for LTOWB for ACD, CPD, or CP2D is ____ days.

21

the transport temperature for LTOWB is ____°C.

1-10°C

A and B hemagglutination titer is ____ for LTOWB.

< 1:256

some BBs only use LTOWB for ____ days and afterwards the unit is spun down, plasma removed and discarded, the RBCs are retained with expiration date as appropriate to anticoagulant preserve.

14

when _____ is given to a patient PRIOR to hospital admission, the patient has a high probability of being mistyped due to transfused blood.

LTOWB

the storage temperature of leuko-reduced (LR) WB is ____°C.

1-6°C

the expiration of leuko-reduced (LR) WB for CPD or CP2D is ____ days.

21

the expiration of leuko-reduced (LR) WB for CPDA-1 is ____ days.

35

QC requirements of leuko-reduced (LR) WB include _____ WBCs and >85% RBC recovery in at least 95% of the units tested.

<5 × 10⁶

usually a ____ WB collection is somewhere between 300 to 404 mLs for a 450mL collection or 333 to 449 mLs for a 500 mL collection.

low volume

only ____ can be made and labeled as low volume.

RBCs

plasma and platelets from low volume units should be discarded. true or false?

true

RBCs in ACD, CPD, or CP2D have a ____ day expiration.

21

RBCs with an additive solution (e.g., Adsol, Nutricel, Optisol) have a ____ day expiration.

42

RBCs in CPDA-1 have a ____ day expiration.

35

leukoreduced RBCs have a ____ day expiration.

42

____% of blood transfusions use RBCs.

98%

____ unit of RBCs raises hemoglobin ____g/dL.

1

____ unit of RBCs raises HCT ~3%.

1

the following are indications for which blood product:

• anemia condition

• less volume to help prevent hyppervolemia

• increased oxygen carrying capacity to vital organs

RBCs

the QC requirements for RBC HCT is ____% for a non additive RBC product.

<80%

additive must be added to RBCs within ____ days of collection when temperature is maintained at 1-6°C.

3

what blood product helps reduce febrile transfusion reactions and TRALI due to lack/reduced number of WBCs and platelets in the product?

LR-RBCs

what is the storage temperature LR-RBCs?

1-6°C

what is the expiration of LR-RBCs in days?

42

what is the transport temperature LR-RBCs?

1-10°C

____ contain RBCs and additive solution with essentially no WBCs or PLTs.

LR-RBCs

for LR-RBCs, _____ WBCs in at least 95% of units tested in required for QC.

<5 × 10⁶

for LR-RBCs, _____% RBC recovery in at least 95% of units tested in required for QC.

>85%

what blood product removes plasma proteins (IgA, IgG, etc.) during washing cycles?

W-RBCs

what blood product removes other accumulated substances present in stored blood (e.g., K+)?

W-RBCs

what is the storage temperature of W-RBCs?

1-6°C

what is the expiration of W-RBCs (open system process)?

24 hours

what is the transport temperature of W-RBCs?

1-10°C

what blood product contains RBCs and saline?

W-RBCs

_____ means the hermetic seal on the original blood bag is broken to gain access into the product.

open system

once the hermetic seal is broken, the expiration date changes from the original outdate to ____.

24 hours

rare phenotypes are ____ for future transfusions (e.g., hr'“ negative, O_h, U negative, etc.).

frozen

autologous blood is often _____.

frozen

high glycerol (____%) is most commonly used in the USA.

40%

low glycerol refers to ____%.

15-20%

F-RBCs must be frozen within ____ days from collection.

6

the storage temperature of _____ F-RBCs is <65°C in a mechanical BB ultra low freezer (usually at -80°C).

high glycerol

the storage temperature of _____ F-RBCs is <165°C in liquid nitrogen vapor OR <196°C in liquid nitrogen in a monitored liquid nitrogen tank.

low glycerol

what is the expiration for F-RBCs?

10 years

the transport temperature for ____ F-RBCs is dry ice.

high glycerol

the transport temperature for ____ F-RBCs is liquid N₂.

low glycerol

prior to the transfusion of F-RBCs, removal of all cryoprotective agent is performed using varying dilutions of saline with dextrose in a process called ____.

deglycerolization

in the USA, F-RBCs must rapidly thaw in a ____°C waterbath before removing the cryoprotective agent.

37°C

thawed and processed F-RBCs are called ____ product.

deglycerolized RBCs

what is the storage temperature for deglycerolized RBCs?

1-6°C

what is the expiration for deglycerolized RBCs (open system process)?

24 hours

what is the transport temperature for deglycerolized RBCs?

1-10°C

what blood product contains RBCs and 0.9% saline with 0.2% dextrose?

deglycerolized RBCs

____ products are made from a whole blood collection using a 2-step differential centrifugation process (low RPM then high RPM).

platelet

what product is used to treat or prevent bleeding from thrombocytopenia and/or platelet functional disorders?

platelet concentrate

platelet concentrate product can raise platelet counts to _____/uL in a 70kg adult.

5000

what is the storage temperature of platelet concentrate?

20-24°C

platelet concentrate requires constant agitation. true or false?

true

what is the expiration of platelet concentrate?

5 days

what is the expiration of platelet concentrate with performance of bacterial detection testing?

7 days

what is the transport temperature of platelet concentrate?

20-24°C

the QC of platelet concentrate is ____ platelets.

>5.5 × 10¹⁰

the QC of platelet concentrate is a pH of ____..

>6.2

what is the expiration of platelet concentrate pooled?

4 hours

the QC requirements of LR-platelet concentrate is ____ WBCs in 95% of the units tested.

<8.3 × 10⁵