Ammonia Testing and Biochemistry Flashcards

Vocabulary flashcards detailing the biochemical assay, reference values, and sample handling procedures for Ammonia.

Ammonia Reference Range

The normal concentration range is $11-32\,\mu mol/L$.

GLDH

Glutamate dehydrogenase, the enzyme used in the reaction involving $\alpha\text{-Kevogluvarate}$, $NH_4^+$, and $NADPH$.

Ammonia Reaction Equation

The biochemical process represented as $\alpha\text{-Kevogluvarate} + NH_4^+ + NADPH \xrightarrow{GLDH} \text{Glutande} + NADP^+ + H_2$.

Initial Sample Handling

The specimen must be put on ice and centrifuged in $<20\,\text{min}$.

Ice Bath Stability

The sample is stable in an ice bath for $3.5\,\text{hours}$.

Frozen Storage

The sample can be kept for a couple of days when frozen.

Reye's Syndrome

A clinical condition associated with the study of ammonia levels based on the lecture notes.