Microbiology Lab exam

what is aseptic technique

a way to handle microbes and limit contamination of media and self

what is A

nose piece

what is B

ocular lense

what is C

stage

what is D

fine adjustment

what is E

illumination dial/ light adjustment

what is the purpose of a streak plate

to get isolated colonies

what mistakes were made with these plates

the needle was not sterilized in the flame in-between each section

Nutrient agar is what type of media

general purpose (neither selective or differential)

is this a gram positive or negative stain

negative

what shape is the bacteria

bacillus

first step of a gram stain

crystal violet

second step of a gram stain

iodine

third step of a gram stain

ethanol

fourth step of a gram stain

safranin

is this a gram positive or negative bacteria

postive

what shape is the bacteria

cocci

what is the green structure

endospore

what genera of bacteria could this be

Bacillus

what tube is a motile bacteria

Tube B

what tube is a nonmotile bacteria

Tube A

what structure confers motility

flagella

What type of media is used to culture fungi

potato dextrose agar

Is this an asexual or sexual spore

Sexual

is this an asexual or sexual spore

asexual

what are unicellular fungi called

yeasts

This is Giardia; is it prokaryote or Eukaryote

eukaryote

High salt agar is

selective media

EMB is

both selective and differential media

Mannitol salt agar is

selective and differential media

blood agar is

enriched media

High salt agar shows

salt tolerance if bacteria grows

EMB shows

purple/ metallic green growth is it is a lactose fermenter

Mannitol salt agar shows

salt tolerance

blood agar shows

type of hemolysis (alpha, beta, gamma)

what are the cleanings called

plaques

Dilution factor is 10 to the -6 o.1ml of phage was used; what is the original concentration of the phage

5\.8 x 10 to the 8 PFU/mL

what is the purpose of top agar

even coverage; allows bacteria to occupy completely

what is the purpose of a control plate

to compare to other plates

where the wells loaded on the L or R

left

what lane would EcoRl (6.2 kb) split into .7kb and 5.5kb

lane 3

Dilution factor is 10 to the -7 and 0.1 of culture was plated, what is the original concentration of the culture

6\.5 x 10 to the 9 CFU/mL

what is the stick called

hockey stick

what is the technique that uses the hockey stick

spread plate

(Phenol red sucrose tube) interpret the tube

acidic; no gas; which is a fermenter with acid production only

(Phenol red sucrose tube) interpret the tube

acidic; gas; which is a fermenter with acid and gas production

(Phenol red sucrose tube) interpret the tube

alkaline; no gas; non fermenter

what carbohydrate is fermented in a Phenol red test

sucrose

what is the purpose of a Durham tube

gas trap

what is this testing for

catalase

what does a positive reaction look like

immediate bubbling

what is this testing for

oxidase

is this a positive or negative result

positive oxidase

what does MIC stand for

minimum inhibitory concentration

what does MBC stand for

minimum bactericidal concentration

What do you interpret MIC from

tubes

what do you interpret MBC from

plates

what is the name of this test

Kirby Bauer/ zone of inhibition

why are there precise rules for the Kirby Bauer test

so it can be used internationally; bacteria resistance can also be compared internationally

if this is staphylococcus epidermis how would the results be interpreted; Penicillin G (10 U)

resistant

if this is staphylococcus epidermis how would the results be interpreted; Streptomycin s10 (10ug)

resistant

if this is staphylococcus epidermis how would the results be interpreted; tetracycline Te30 (30 ug) 

susceptible

what type of substate is tirbutyrin

lipid

what enzyme is it testing for

lipase production

How would the top plate be interpreted

positive for lipase production

how would the bottom plate be interpreted

negative; no lipase production

what was added to the plates to allow interpretation

iodine

starch agar plates; what enzyme is being tested

amylase production

how would you interpret the top plate

postive for amylase production

how would you interpret the bottom plate

negative for amylase production

skim milk agar; what enzyme is being tested

caseinase production

how would you interpret the top plate

positive for caseinase production; clearing around line of bacteria

how would you interpret the bottom plate

negative for caseinase production; no clearing

gelatin tubes; what enzyme is being tested

gelatinase

how would you interpret tube A

solid; negative for gelatinase production

how would you interpret tube B

liquid; positive for gelatinase production

citrate slants; what is being tested

citrate production

how would you interpret tube A

negative for citrate production

how would you interpret tube B

postive for citrate production

tryptone tubes; what is this testing for

ability to degrade amino acids

how would you interpret tube A

positive for indole production (a)

how would you interpret tube B

positive for indole production (b)

what does a negative indole test look like

stays the same color/ yellowish

what is coliform

gram negative non spore forming rods that ferment lactose to acid and gas

what does MPN stand for

most probable number

What tubes are positive for coliforms

3,4,5, and 1mL

what is the result of this assay

12 MPN

what immune cell is this

lymphocyte

what are the results of this gram stain

gram negative bacilli

what are the results of this oxidase test

postive

what are the results of this MR test

postive

what are the results of this gram stain

gram postive bacilli

what are the results of this spore test

postive endospore forming

what are the results for this amylase test

negative for amylase production; no clearing

what are the results of this MR test

positive for glucose fermentation

what are the results of this MR test

negative for glucose fermentation

what are the results of this VP test

positive for the presence of acetoin

what are the results of this VP test

negative for the presence of acetoin

what plate section is used to determine the MBC

section 1

what tube is used to determine the MIC

tube 2