Studied by 5 people
what is aseptic technique
a way to handle microbes and limit contamination of media and self
what is A
nose piece
what is B
ocular lense
what is C
stage
what is D
fine adjustment
what is E
illumination dial/ light adjustment
what is the purpose of a streak plate
to get isolated colonies
what mistakes were made with these plates
the needle was not sterilized in the flame in-between each section
Nutrient agar is what type of media
general purpose (neither selective or differential)
is this a gram positive or negative stain
negative
what shape is the bacteria
bacillus
first step of a gram stain
crystal violet
second step of a gram stain
iodine
third step of a gram stain
ethanol
fourth step of a gram stain
safranin
is this a gram positive or negative bacteria
postive
what shape is the bacteria
cocci
what is the green structure
endospore
what genera of bacteria could this be
Bacillus
what tube is a motile bacteria
Tube B
what tube is a nonmotile bacteria
Tube A
what structure confers motility
flagella
What type of media is used to culture fungi
potato dextrose agar
Is this an asexual or sexual spore
Sexual
is this an asexual or sexual spore
asexual
what are unicellular fungi called
yeasts
This is Giardia; is it prokaryote or Eukaryote
eukaryote
High salt agar is
selective media
EMB is
both selective and differential media
Mannitol salt agar is
selective and differential media
blood agar is
enriched media
High salt agar shows
salt tolerance if bacteria grows
EMB shows
purple/ metallic green growth is it is a lactose fermenter
Mannitol salt agar shows
salt tolerance
blood agar shows
type of hemolysis (alpha, beta, gamma)
what are the cleanings called
plaques
Dilution factor is 10 to the -6 o.1ml of phage was used; what is the original concentration of the phage
5.8 x 10 to the 8 PFU/mL
what is the purpose of top agar
even coverage; allows bacteria to occupy completely
what is the purpose of a control plate
to compare to other plates
where the wells loaded on the L or R
left
what lane would EcoRl (6.2 kb) split into .7kb and 5.5kb
lane 3
Dilution factor is 10 to the -7 and 0.1 of culture was plated, what is the original concentration of the culture
6.5 x 10 to the 9 CFU/mL
what is the stick called
hockey stick
what is the technique that uses the hockey stick
spread plate
(Phenol red sucrose tube) interpret the tube
acidic; no gas; which is a fermenter with acid production only
(Phenol red sucrose tube) interpret the tube
acidic; gas; which is a fermenter with acid and gas production
(Phenol red sucrose tube) interpret the tube
alkaline; no gas; non fermenter
what carbohydrate is fermented in a Phenol red test
sucrose
what is the purpose of a Durham tube
gas trap
what is this testing for
catalase
what does a positive reaction look like
immediate bubbling
what is this testing for
oxidase
is this a positive or negative result
positive oxidase
what does MIC stand for
minimum inhibitory concentration
what does MBC stand for
minimum bactericidal concentration
What do you interpret MIC from
tubes
what do you interpret MBC from
plates
what is the name of this test
Kirby Bauer/ zone of inhibition
why are there precise rules for the Kirby Bauer test
so it can be used internationally; bacteria resistance can also be compared internationally
if this is staphylococcus epidermis how would the results be interpreted; Penicillin G (10 U)
resistant
if this is staphylococcus epidermis how would the results be interpreted; Streptomycin s10 (10ug)
resistant
if this is staphylococcus epidermis how would the results be interpreted; tetracycline Te30 (30 ug)
susceptible
what type of substate is tirbutyrin
lipid
what enzyme is it testing for
lipase production
How would the top plate be interpreted
positive for lipase production
how would the bottom plate be interpreted
negative; no lipase production
what was added to the plates to allow interpretation
iodine
starch agar plates; what enzyme is being tested
amylase production
how would you interpret the top plate
postive for amylase production
how would you interpret the bottom plate
negative for amylase production
skim milk agar; what enzyme is being tested
caseinase production
how would you interpret the top plate
positive for caseinase production; clearing around line of bacteria
how would you interpret the bottom plate
negative for caseinase production; no clearing
gelatin tubes; what enzyme is being tested
gelatinase
how would you interpret tube A
solid; negative for gelatinase production
how would you interpret tube B
liquid; positive for gelatinase production
citrate slants; what is being tested
citrate production
how would you interpret tube A
negative for citrate production
how would you interpret tube B
postive for citrate production
tryptone tubes; what is this testing for
ability to degrade amino acids
how would you interpret tube A
positive for indole production (a)
how would you interpret tube B
positive for indole production (b)
what does a negative indole test look like
stays the same color/ yellowish
what is coliform
gram negative non spore forming rods that ferment lactose to acid and gas
what does MPN stand for
most probable number
What tubes are positive for coliforms
3,4,5, and 1mL
what is the result of this assay
12 MPN
what immune cell is this
lymphocyte
what are the results of this gram stain
gram negative bacilli
what are the results of this oxidase test
postive
what are the results of this MR test
postive
what are the results of this gram stain
gram postive bacilli
what are the results of this spore test
postive endospore forming
what are the results for this amylase test
negative for amylase production; no clearing
what are the results of this MR test
positive for glucose fermentation
what are the results of this MR test
negative for glucose fermentation
what are the results of this VP test
positive for the presence of acetoin
what are the results of this VP test
negative for the presence of acetoin
what plate section is used to determine the MBC
section 1
what tube is used to determine the MIC
tube 2
Note 
Flashcard (25)