BIO 320 Operons and Horizontal Gene Transfer

Which statements is/are false?

- Beta-galactosidase separates lactose and glucose.
- Permease allows beta-galactosidase into the cell
- Lactose is the inducer of the lac operon
- Bacteria want to make beta-galactosidase when lactose is present
- Lactose changes the conformation (shape) of the lac repressor.

Beta-galactosidase separates lactose and glucose.

Permease allows beta-galactosidase into the cell

Which of the following is/are relevant to the lac operon?

inducer, co-repressor, repressor

inducer, repressor

Which of the following is/are relevant to the trp operon?

inducer, co-repressor, repressor

co-repressor, repressor

T/F: Beta-galactosidase breaks lactose into glucose and fructose.

False, beta galactosidase breaks lactose into glucose and galactose

Which statements are true?

- An operon is a series of genes under the control of a single promoter.
- An inducer caused a repressor to work.
- When tryptophan is in the media, bacteria would like to make more tryptophan, and expression of the relevant genes is upregulated (turned on)
- When lactose is present, bacteria would like to make β-galactosidase, The repressor will not bind the operator.
- In the absence of glucose decreased levels of cAMP prevent CAP from binding to DNA

An operon is series of genes under the control of a single promoter.

When lactose is present, bacteria would like  to make β-galactosidase, The repressor will not bind the operator.

Which of the following are relevant to the tryptophan operon?

corepressor, inducer, RNA polymerase

corepressor, RNA polymerase

T/F: Bacteria do not want to induce (turn on) the lac operon in the absence of lactose because it would be a waste of resources and energy.

true

Which statements is/are true?

- Together glucose and the catabolite activator protein (CAP) protein bind to DNA.
- Increases in cAMP cause glucose levels to go down and the two are therefore inversely related.
- Beta-galactosidase be made only if glucose is absent.
- The lac and trp operons would cease to function properly if their repressors were no longer made constitutively

Beta-galactosidase be made only if glucose is absent. 

The lac and trp operons would cease to function properly if their repressors were no longer made constitutively

T/F: RNA polymerase binds to the promoter region. In bacteria this is at the -35 region.

True

Which statement best describes gene expression?

- RNA being made (copied) from DNA
- DNA being made (copied) from RNA
- Proteins being made from DNA
- Proteins being made from RNA

- RNA being made (copied) from DNA

Which statement(s) are true?

- An operon is a series of promoters under the control of a single gene.
- Tryptophan is made by bacteria cells when it can be obtained from the surroundings.
- Tryptophan can act as a corepressor.
-The trp repressor bind to the operator region in the absence of tryptophan.
- Tryptophan is an amino acid.

Tryptophan can act as a corepressor.

Tryptophan is an amino acid.

Match the following

- Transfer is performed by viruses
- Plasmid is transferred via a F pilus
- Naked DNA is taken up by bacteria

A. Transformation
B. Transduction
C. Conjugation

Transformation, Naked DNA is taken up by bacteria

Transduction, Transfer is performed by viruses,

Conjugation, Plasmid is transferred via a F pilus,