(CIE A2 Biology) Desired gene isolation (based on SaveMyExams revision notes)

0.0(0)
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/9

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

10 Terms

1
New cards

Restriction Endonucleases

A class of enzymes found in bacteria that cut viral genetic material and isolate genes from donor DNA.

<p>A class of enzymes found in bacteria that cut viral genetic material and isolate genes from donor DNA.</p>
2
New cards

cDNA (complementary DNA)

Single-stranded DNA synthesized from mRNA using reverse transcriptase.

<p>Single-stranded DNA synthesized from mRNA using reverse transcriptase.</p>
3
New cards

Sticky Ends

DNA fragments with overhanging sequences that facilitate easier insertion of genes into other DNA.

<p>DNA fragments with overhanging sequences that facilitate easier insertion of genes into other DNA.</p>
4
New cards

Blunt Ends

DNA fragments with straight cuts in the sugar-phosphate backbone, making them less efficient for insertion.

<p>DNA fragments with straight cuts in the sugar-phosphate backbone, making them less efficient for insertion.</p>
5
New cards

Reverse Transcriptase

An enzyme that catalyzes the conversion of mRNA into cDNA.

6
New cards

DNA Polymerase

An enzyme that converts single-stranded cDNA into double-stranded DNA.

7
New cards

Artificial Gene Synthesis

The process of creating genes using computers to generate nucleotide sequences based on known genetic codes.

<p>The process of creating genes using computers to generate nucleotide sequences based on known genetic codes.</p>
8
New cards

Retroviruses

Viruses that use reverse transcriptase to transcribe their RNA into DNA.

9
New cards

Nucleotide Sequence

A specific order of nucleotides in a DNA or RNA molecule that encodes for genes.

10
New cards

Vector

A vehicle, such as a plasmid, used to artificially insert genes into an organism.