Polymerase Chain Reaction (PCR) and Netiquette

Flashcards created to aid in understanding the Polymerase Chain Reaction (PCR) and basic internet etiquette.

What are the key steps in the Polymerase Chain Reaction (PCR) process?

Denature DNA, Anneal primers, Extend primers.

What is the purpose of primers in PCR?

Primers are short sequences that provide a starting point for DNA synthesis.

At what temperature does DNA denaturation occur in PCR?

Between 92–95°C.

What is the temperature range for primer annealing in PCR?

45-65°C.

What does Taq polymerase do in the PCR process?

It synthesizes new DNA strands by adding nucleotides to the primers.

How many product molecules are generated in the first cycle of PCR?

2 product molecules.

What is the end of a PCR primer that can be extended by polymerase?

The 3' end.

What is netiquette?

Internet etiquette that ensures a safe and friendly online environment.

List two netiquette rules for online class discussions.

Keep your microphone on mute and listen attentively.

What is Reverse Transcription PCR (RT PCR) used for?

To amplify RNA sequences, often in diagnostic applications.