Culturing microbes (required practical): Cell biology: Biology: GCSE (9:1)

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21 Terms

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Binary fission

How bacteria asexually reproduce

<p>How bacteria asexually reproduce</p>
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Colonies

A visible mass of many bacteria

<p>A visible mass of many bacteria</p>
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Nutrient broth solution

A liquid food culture media to grow bacteria

<p>A liquid food culture media to grow bacteria</p>
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Agar gel plates

A solid food culture media to grow bacteria

<p>A solid food culture media to grow bacteria</p>
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Aseptic technique

A way of preventing contamination when growing bacteria

<p>A way of preventing contamination when growing bacteria</p>
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Petri dish

A glass or plastic dish where bacteria are grown on agar

<p>A glass or plastic dish where bacteria are grown on agar</p>
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Inoculating loop

A sterile looped piece of wire which can transfer bacteria between culture media

<p>A sterile looped piece of wire which can transfer bacteria between culture media</p>
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Incubator

A machine with a set temperature to grow bacteria

<p>A machine with a set temperature to grow bacteria</p>
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Contamination

When unwanted bacteria or other microorganisms grow in the culture media

<p>When unwanted bacteria or other microorganisms grow in the culture media</p>
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Culture media

A liquid or solid food source to grow bacterial cultures in

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Antiseptic

A substance which kills bacteria and other microorganisms

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Antibiotic

A medication which is used to kill specific bacteria

<p>A medication which is used to kill specific bacteria</p>
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Sterilisation

A method of removing microorganisms from surfaces usually by means of chemicals or heat

<p>A method of removing microorganisms from surfaces usually by means of chemicals or heat</p>
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Disinfectants

A substance which prevents the growth of bacteria and other microorganisms

<p>A substance which prevents the growth of bacteria and other microorganisms</p>
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Zone of inhibition

An area of media where bacteria cannot grow that shows the effectiveness of a disinfectant or antibiotic

<p>An area of media where bacteria cannot grow that shows the effectiveness of a disinfectant or antibiotic</p>
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Diameter or area of clear zone

The dependent variable or factor that is measured in this experiment to investigate the effect of disinfectants

<p>The dependent variable or factor that is measured in this experiment to investigate the effect of disinfectants</p>
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Type or concentration of disinfectant

The independent variable or factor that is changed in this experiment to investigate the effect of disinfectants

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Temperature, time, volume, concentration

Possible variables that can be controlled to make sure the experiment is a fair test

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Experimental procedure

Multiple sterile discs should be dipped into different disinfectants or different concentrations of disinfectant, after which they should be carefully placed on agar and incubated with bacteria

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Expected results

The more effective the disinfectant, the larger the area of the zone of inhibition

<p>The more effective the disinfectant, the larger the area of the zone of inhibition</p>
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Control sample

A disc soaked with water and no disinfectant on can be used as a control to compare with discs that have been dipped in disinfectant

<p>A disc soaked with water and no disinfectant on can be used as a control to compare with discs that have been dipped in disinfectant</p>