MLT 110- Microscopes

4x lens

lowest power objective lens, known as the scanning lens.

10x lens

low power objective lens, second smallest.

40x lens

high power objective lens

100x lens

highest power objective lens, used with immersion oil

How do you find the total magnification?

Ocular lens multiplied by the in-use objective lens

Magnification of the ocular lens?

10x

Focal length

The distance between the objective lens and the specimen being observed

Resolution

How well a microscope distinguishes between two points that are close together

Interpupillary distance

The distance between the ocular lenses

Parfocal

After a specimen is focused, objective lenses can be changed with minimal refocusing

Working distance

The space between the objective lens and specimen surface when focused

length of the oil lens focal length

Short

What is used to clean the exterior of your microscope?

Kimwipe

What must you follow a 10% bleach clean with?

Alcohol

Brightfield microscope

Has a light background and a dark specimen

Darkfield microscope

Has a light specimen and a dark background

Phase contrast microscope

Increases the contrast in unstained specimens

Fluorescence microscope

For fluorescence

Polarized microscope

For crystal identification in bodily fluids

Digital microscope

For locating and preclassifying blood cells, makes sharing the image easy

Electron microscope

Has a greater magnification and resolution than conventional microscopes

Scanning electron microscope

Has the greatest magnification and resolution

How do you clean the glass portions of a microscope?

Lens paper and cleaner

What is the most common type of microscope?

Brightfeild

Which shows more definition, scanning electron or electron microscopes?

Scanning electron

Which objective lens requires the most light for use?

Oil