Lesson 19 - Column Chromatography

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10 Terms

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Column Chromatography

A technique used to purify compounds by packing stationary phases into a vertical glass column.

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Stationary Phase

The phase in a chromatography process that remains fixed in the column, typically silica gel or alumina.

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Mobile Phase

The solvent that moves through the column, carrying the sample with it during the separation process.

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Elution

The process of washing a compound through a column using a solvent.

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Silica Gel

A common stationary phase used in column chromatography that is hydrophilic and polar.

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Fraction Collection

The practice of collecting the eluted samples at different time intervals during column chromatography.

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Polar Compounds

Substances that have a significant charge separation, causing them to interact more strongly with the stationary phase.

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Non-polar Compounds

Substances that do not have significant charge separation and tend to elute faster in column chromatography.

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Sorbent

The material in which solutes adsorb in column chromatography, typically silica gel or alumina.

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Particle Size

The average size of the stationary phase particles, which affects the separation of different molecular sizes in column chromatography.