MIC 102 Lecture 1 - Intro

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Microscopy Basics

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7 Terms

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Light Microscopy

Magnifies up to 1000x ; resolve structures around 200 nm apart ; Brightfield, Phase contrast, and Fluorescence are common techniques

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Brightfield

To view pigmented or stained specimens (High Contrast)

<p>To view pigmented or stained specimens (High Contrast) </p>
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Phase Contrast

To view non-pigmented (low contrast) specimens

<p>To view non-pigmented (low contrast) specimens</p>
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Fluorescence

To view cells/ structures labeled with a fluorochrome

<p>To view cells/ structures labeled with a fluorochrome </p>
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Electron Microscopy

Magnifies up to 500,000x; can resolve structures, estimated 0.1 nm apart; including Scanning, Transmission

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Scanning EM

Scans the exterior of a specimen to reveal the topography and fine details; requires processing of the specimen

<p>Scans the exterior of a specimen to reveal the topography and fine details; requires processing of the specimen </p>
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Transmission EM

Used to view the internal structures of (embedded, sliced, stained) specimens or view small specimens

<p>Used to view the internal structures of (embedded, sliced, stained) specimens or view small specimens </p>