Granulocyte series

Granulopoiesis

called to Process of granulocyte (neutrophil, eosinophil, basophil) development and maturation.

Changes from blue (basophilic) to pink (acidophilic) as cell matures.

Cytoplasmic change during granulopoiesis

Becomes coarser and more clumped as the cell matures.

Nuclear chromatin change during granulopoiesis

Granules appear progressively as cell matures (primary - secondary - tertiary)

Granules appearance during granulopoiesis

Cell size decreases as maturation progresses

Cell size change during granulopoiesis

Myeloblast, Promyelocyte, Myelocyte.

Mitotically active stages

Occurs in the bone marrow.

Location of granulopoiesis

Myeloblast

Earliest recognizable precursor of granulocytes.

15 - 20 um

Myeloblast size

blue to pink upon matiration

myeloblast cytoplasm

Round or Oval

Myeloblast nucleus

4:1

Myeloblast Nucleus to Cytoplasm Ratio

3-5

Myeloblast Nucleoli

None

Granules of Myeloblast

15-21um

Promyelocyte Size

Promyelocyte N:C rat

Blue

Promyelocyte Cytoplasm

Round

Promyelocyte Nucleus

appearance of primary granules ( azurophilic or non specific granules )

Granules of Promyelocyte

true

TF: Granules look similar and cannot differentiate cell type yet (neutrophil/eosinophil/basophil).

12-28 um

Myelocyte size

absent

Myelocyte Nucleoli

Appearance of secondary ( specific ) granules

Myelocyte Granules

True

TF: In myelocyte you can identify cell type

Pink to lilac granules

Neutrophilic Myelocyte color

Red to Orange granules

Eosinophilic Myelocyte

Blue to Black granules ( largest granules )

Basiphilic Myelocyte

F, Basophils are bigger than eosinophils.

TF: Eosinophilia is bigger than basophil

true

TF: Myelocyte is still capable of mitosis

Juvenile Cell

Other name of Metamyelocyte

10-15um

Metamyelocyte size

Kidney or bean shaped

Metamyelocyte Nucleus

Less than the width of the nucleus

Metamyelocyte Identation

Apperance of tertiary granules

Granules of Metamyelocyte

Metamyelocyte

the first stage to release into peripheral circulation

Metamyelocyte

the stage that cannot be divide anymore

Stab and Staff Cell

Other names for Band cell

S or C shaped

Nucleus of Band cell

More than the width of the nucleus

Identation of band cell

Normally found in small amounts in peripheral blood

Presence of Band cell

Last stage with production of tertiary granules

Granules of band cell

true

TF: Immature neutrophil form before becoming segmenter.

Segmented ( Mature ) Granulocyte

Fully mature granulocyte with separated nuclear lobes connected by thin filaments.

Fully mature granulocyte with separated nuclear lobes connected by thin filaments.

characteristics of Segmented ( mature ) granulocyte

9-15 um

Neutrophil size

2-5 lobes ( purple )

Neutrophil nucleus

purple

color of neutrophil nucleus

Phagocytosis and acute inflammation.

Neutrophil function

9-15um

Eosinophil size

bilobed ( 2 lobes )

Eosinophil Nucleus

Large red-orange granules

Granules of Eosinophil

Allergic response and parasitic defense.

Eosinophil function

true

TF: In eosinophil the Granules are larger than neutrophil

Similar range (99-15um)

Basophil size

Blue-black, largest granules

Basophil granules

Releases histamine and heparin during inflammation/allergy.

Basophil function

histamine and heparin

basophil releases what during inflammation and allergy

Band: Nucleus is curved but not separated into lobes (continuous).

Segmenter: Nucleus divided into distinct lobes connected by thin filaments.

How to differentiate Band cell from Segmenter

Metamyelocyte (Juvenile).

Stage where tertiary granules appear

Metamyelocyte

Stage first released into circulation

Myeloblast, Promyelocyte, Myelocyte.

Stage that still undergoes mitosis

Promyelocyte

Stage with primary granules

Myelocyte

Stage with secondary granules

Metamyelocyte (and Band).

Stage with tertiary granules

False - Size decreases with maturation.

T/F: Granulocyte size increases as it matures.

False - They have the biggest granules.

T/F: Basophils have the smallest granules.

T/F: Myeloblast is the earliest stage of granulopoiesis.

True

True

T/F: Myelocyte can still divide.

False - It is curved (S- or C-shaped), not yet separated.

T/F: Band cell nucleus is segmented.

True

TF: Segmenter is the mature granulocyte.

False - They are non-specific (azurophilic) granules.

T/F: Primary granules are specific granules.