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What are the 3 functions of blood
transportation, defense, regulation
What makes up whole blood
plasma (55%) and cellular elements (45%)
How much plasma is water
90%
Aside from water, what else makes up plasma
proteins, vitamins, enzyme, waste, carbs, hormones, lipids
What gives RBC its color
iron/hemoglobin
What part of whole blood helps fight off "invasions"
WBC
What part of whole blood controls bleeding?
platelets
What is a hematology test that determines the number of and type of blood cell present in a sample/patient
CBC
What does packed cell volume check
% of rbc
Do blue hematocrit tubes use an anticoagulant or not?
no anticoagulant
Do red hematocrit tubes use an anticoagulant or not?
yes
You centrifuge a sample at for _____ min and ____ rpm
5 min, 3000 rpm
What happens when you shake a tube of blood?
hemolysis
You use the plasma layer to determine
total proteins
Normal color of plasma
clear or straw colored
Lipemic plasma will be
cloudy
Icteric plasma will be
yellow
Hemolytic plasma will be
pink/red
Lipemic and hemolytic plasma will be
red and cloudy
Yellow pigment is due to excess ________ in blood
bilirubin
What does the buffycoat consist of
thrombocytes and leukocytes
Normal canine PCV
37-55%
Normal feline PCV
30-45%
Increased PCV levels can indicate
dehydration
Decreased PCV levels can indicate
anemia
A common blood protein manufactured in the liver that helps maintain osmotic pressure
albumin
A common blood protein that plays a role in the immune system
globulin
A common blood protein that aids in clotting
fibrinogen
What is the unit for total protein?
g/dL
What is a normal canine TP value?
5.4-7.5 g/dL
What is a normal feline TP value?
5.7-7.6 g/dL
Where is the spleen located?
top L side of abdomen
What stores and produces RBC during fetal development
the spleen
How to get the RBC count
PCV/6
How to get the Hgb count
PCV/3
How to get the MCV count
PCV/RBC( 10)
How to get MCH
Hgb/RBC (10)
How to get MCHC
Hgb/PCV (100)
What color top do you take blood out of when making a blood smear
purple top
What angle should the spreader slide be at when making a blood smear?
30-40 degree angle
Body parts of a blood smear
body
monolayer
feathered edge
What is the thickest part of the blood smear
body
What is the thinnest part of the blood smear
feathered edge
What part of the blood smear is NOT used for diagnostic imaging?
body
Where is majority of diagnostic imaging preformed on a blood smear?
the monolayer
What part of the blood smear can show baby heartworm? (microfilaria)
feathered edge
What is the color order you stain the slides
blue
red
purple
