J-couplings, nD-NMR experiments and backbone resonance assignment not done

what kind of axes do nD NMR spectra have

directly detected and indirectly detected

1D NMR experiment of nucleus X

excite sample with appropriate radio frequency pulse

detect FID with a detector, show how amplitude of signal decays over time

do fourier transform of FID

2D NMR experiment of nucleu X and Y

detect resonance frequency from transfer of magnetism of X to Y, then Y to X, then do a fourier transform to give chemical shift

do not let frequency of Y run out as this is the starting magnetisation for X

repeat with different time lengths for Y

what are through-bond J couplings used for

transfer magnetism between covalently bonded nuclei

what are 2D HX heteronuclear experiments used for

to generate spectral fingerprints of proteins

what are 2D (1H, 15N) HSQC-type spectra used for

characterising

whether a protein is folded or not

binding interactions

molecular dynamics

domain-domain interactions in multidomain proteins (excludes proline, N-terminal amine and side chain amines)

a larger J coupling means

a more efficient transfer