Practicum 2: Gram-Positive Rods

bacillus cereus is catalase ____.

positive

listeria monocytogenes is catalase ____.

positive

erysipelothrix rhusiopathiae is catalase ____.

negative

corynebacterium diphetheriae is catalase ____.

positive

bacillus cereus is oxidase ____.

variable

listeria monocytogenes is oxidase ____.

negative

erysipelothrix rhusiopathiae is oxidase ____.

negative

corynebacterium diphtheriae is oxidase ____.

negative

large rods in chains:

bacillus cereus

tiny rods in clusters and pairs:

listeria monocytogenes

filamentous, curly rods:

erysipelothrix rhusiopathiae

palisades, club-shaped:

corynebacterium diphtheriae

weak beta hemolysis:

listeria monocytogenes; corynebacterium diphtheriae

weak hemolysis (either alpha or beta):

erysipelothrix rhusiopathiae

strong beta hemolysis:

bacillus cereus

K/A (red slant, yellow butt from glucose fermentation only):

bacillus cereus; corynebacterium diphtheriae

A/A (yellow slant, yellow butt due to glucose AND lactose fermentation):

listeria monocytogenes

K/K (red slant, red butt due to no fermentation with black precipitate due to H2S production):

erysipelothrix rhusiopathiae

esculin hydrolysis POSITIVE (black esculin slant):

bacillus cereus; listeria monocytogenes

esculin hydrolysis NEGATIVE (no color change to esculin slant):

erysipelothrix rhusiopathiae; corynebacterium diphtheriae

what bacteria is NOT motile at 37°C but motile at room temperature?

listeria monocytogenes

which bacteria is motile?

bacillus cereus

which bacteria is NOT motile?

erysipelothrix rhusiopathiae; corynebacterium diphtheriae