Siemens PEP: Immunoassay

substance or particle that the body considers to be foreign or potentially harmful:

antigen

Y-shaped protein produced by the body as an immune response against antigens:

antibody

used in the detection and monitoring of myocardial infarction or heart failure:

cardiac markers

troponin I, CK-MB, myoglobin, BNP, D-Dimer are examples of:

cardiac markers

chemical substances formed in one organ and carried to another organ on which they exert physiological effects such as growth and metabolism:

hormones

TSH, T4, and T3 are examples of ____ hormones.

thyroid

FSH, LH, and total hCG are examples of ____ hormones.

fertility

cortisol is an example of a(n) ____ hormone.

adrenal

insulin is an example of a(n) ____ hormone.

pancreatic

proteins that are used in the detection and monitoring of certain cancers:

cancer markers

what cancer marker is associated with prostate cancer?

PSA

what cancer marker is associated with breast cancer?

CA 15-3

what cancer marker is associated with ovarian cancer?

CA 125

what cancer marker is associated with pancreatic cancer?

CA 19-9

____ testing uses antibodies or antigens to isolate and detect specific analytes in the blood.

immunoassay

which immunoassay testing method refers to reactions produce a colored end-product?

photometry

specific photometer that uses a filter to select the desired wavelength of light:

colorimeter

specific photometer that is more sophisticated that uses a monochromator consisting of a prism or diffraction grating to split the light into various wavelengths and then a slit to select the wavelength used in measurement:

spectrophotometer

which immunoassay testing method refers to end products that can produce fluorescence?

fluorometry

which immunoassay testing method refers to measuring cloudiness (not color)?

turbidimetry

which immunoassay testing method refers to the end product being particulate and produces cloudiness?

turbidimetry

which immunoassay testing method refers to light being reduced because it is scattered, reflected, or absorbed by the mixture?

turbidimetry

which immunoassay testing method refers to relatively clear solutions where light is weakly scattered because of a low concentration of particles or extremely small particles compared with the wavelength of incident light?

nephelometry

which immunoassay testing method refers to containing many particles or particles that are large compared with wavelength of incident light?

turbidimetry

testing for IgG is an example of which immunoassay method?

nephelometry

this is the Heidelberger-Kendall Curve. in the (color: white, black, grey) ____ zone, there is excess antibody.

white

this is the Heidelberger-Kendall Curve. in the (color: white, black, grey) ____ zone, there is an equivalence zone (unstable area) where there is equal amount of antibodies and antigens.

black

this is the Heidelberger-Kendall Curve. in the (color: white, black, grey) ____ zone, there is excess antigen.

grey

which immunoassay testing method refers to a special detection method that is used for chemical reactions that produce photons of light when the reaction occurs?

chemiluminescence

which immunoassay testing method refers to no incident source light since the chemical reaction itself produces the light that is measured?

chemiluminescence

what form of chemiluminescence refers to the analyte in the sample is bound to the antibody site, then labeled antibody is bound to the analyte?

sandwich binding

in sandwich binding, the analyte concentration in the sample and the light emission have a(n) ____ relationship?

direct

what form of chemiluminescence refers to the labeled antigen competing with sample antigen for limited antibody binding sites on the solid phase?

competitive binding using labeled antigen

in competitive binding using labeled antigen, the analyte concentration in the sample and the light emission have a(n) ____ relationship?

inverse

what form of chemiluminescence refers to the sample antigen and the solid phase bound antigen compete for limited binding sites on the labeled antibodies?

competitive binding using labeled antibody

in competitive binding using labeled antibody, the analyte concentration in the sample and the light emission have a(n) ____ relationship?

inverse

what form of chemiluminescence refers to detecting and quantifying specific IgG or IgM human antibodies in patient samples?

antibody capture

in antibody capture, the analyte concentration in the sample and the light emission have a(n) ____ relationship?

direct