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endocytosis
ingestion of large particles
exocytosis
secretion of large particles
phagocytosis
ingesting food
pinocytosis
ingesting liquid
isotonic
equal rates of osmosis
hypotonic
bloated cell (more solute inside cell than outside)
hypertonic
shriveled cell (less solute inside cell than outside)
peripheral protein
type of membrane protein that temporarily attaches to lipid
integral protein
types of membrane protein that permanently attaches to cell membrane
anabolic reactions
endergonic; products have more free energy than reactants ; non spontaneous
catabolic reactions
exergonic; products have less free energy than reactants; spontaneous
rate of reaction is influenced by
pH, temperature, enzyme & substrate concentrations, activators and inhibitors
activators
stabilize active form and help substrate fit
coenzyme
organic activator
cofact
inorganic activator
inhibitors
stabilize the inactive form
competitive inhibitor
binds directly to the active site and competes directly
allosteric/noncompetitive inhibitor
binds to a different part of the enzyme other than the active site, causing the active site to change shape
stages of cellular respiration
glycolysis, prep rxn, krebs/citric acid cycle, oxidative phosphorylation
where does glycolysis occur?
cytoplasm
net gain of glycolysis?
2 ATP and 2 NADH
fermentation
when pyruvate at the end of glycolysis is converted into ethanol in bacteria and llactate in animal cells; anaerobic process
where does prep rxn occur?
mitochondria
pyruvate oxidation
pyruvate → 2 acetyl CoA
where does krebs cycle occur?
mitochondrial matrix
net gain of krebs cycle?
2 ATP, 6NADH, 2FADH2
where does oxidative phosphorylation occur?
mitochondrial matrix
what does the etc do?
uses all 10 NADH and 2 FADH2 to pump H+ from low to high concentration
final electron acceptor in etc?
oxygen
chemiosmosis
makes 32-34 ATP; diffuses H+ through ATP synthase
phosphofructokinase/pfk
allosteric enzyme that controls rate of glycolysis and krebs cycle; inhibited by ATP
photosynthesis rxn
6CO2 + 6H2O + light energy → C6H12O6 + 6O2
light-dependent reaction
uses photosystems II and I to absorb sunlight and accept electrons; makes 1 net ATP
calvin cycle
occurs in stroma; NADPH, ATP, and CO2 from light rxn is used to build G3P