HGP

HGP was launched in the year

1990

Cost of sequencing per bp and total

3$

9 billion $

Organisations that were part of it

Coordinated by US department of Energy and the national institute of health

During early years Wlcome trust (UK) was major partner

Others: Japan, France, Germany, china

Non human model organism

Bacteria

Yeast

Caenorhabditis elegans

Drosophila

Plants( rice and Arabidopsis)

Caenorhabditis elegans is

Free living

Non pathogenic

Nematode

Two approaches were

EST

Sequence Annotation

YAC & BAC are

Vectors used

Automated DNA sequences worked on principle of

Fredrick Sanger

(Also developed method for determination of amino acid sequences in proteins)

Chromosome 1 sequence completed in ?

May 2006

Human genome contains _______ bp

3164.7 million

Largest human gene? _____ bp

Dystrophin

2.4 million

Genes in chr 1 and chr. Y

2968

231

SNPs occur in humans at ____ locations

1.4 million

____ is the basis of genetic mapping and Dna fingerprinting

Polymorphism

DNA fingerprinting developed by

Alec Jeffreys initially

Jeffery used ____DNA as probe called as _____ and used ______ hybridization using radio Labled probe

Satellite

VNTR

Southern blot

VNTR belongs to a class of stellite dna k/a

Mini satellite

Size of VNTR

0.1-20 kb

Sensitivity of fingerprinting increased by using

PCR