MOD9 Enterobacterales ID

IMViC

indole

methyl red

voges-proskauer

citrate

series of biochemical tests to ID Enterobacterales

API 20E

analytical profile index

IDs Enterobacterales and some non-Enterobacterales

Vitek uses

combination of ID testing and susceptibility testing for non-fastidious organisms

MALDI-TOF MS

matrix assisted laser desorption/ionization

time of flight

mass spectrometry

target slide

three 4×4 acquisition groups + calibration spot for E.coli ATCC8739 in center

calibrant serves as internal control for each acquisition group → not there, entire group is invalid

monolayer of pure/isolated org from fresh culture applied to spots (16) → spot quality most important

matrix

added after an isolate has been added to each spot

absorbs laser energy → even ionization across entire spot

formic acid

facilitates ID of yeast and difficult to ID organisms

breaks down cell wall, exposes more proteins for ionization