Test Reagents

dimethyl-p-phenylene diamine hydrochloride

Used for oxidase test, this reagent is oxidized by cytochrome c oxidase and turns dark blue to black

Sulfanillic acid

Nitrate I

Dimethyl alpha naphthylamine

Nitrate II

Barrits reagents?

Alpha Naphthol(VPI) and KOH (VPII)

Brilliant green?

Is a inhibitor in shigella salmonella agar and Bismuth sulfite agar

Malachite green?

Primary dye used in spore staining, the schaffer fullton methiod

Carbol Fuchsin?

The primary dye used in the zeel nielson or acid fast stain of mycobacterium leprae/tuberculosis

Neutral red?

Indicator used in MacConkey and Shigella Salmonella

Turns pink/red when exposed to acids

Phenol red?

Is used in PR sugar broths/mannitol salt agar/urea
Turns yellow:acid
Remains red:neutral
Turns pink: NH3/Base

10% FeCL3

Reacts with Phenyl Pyruvic acid, a product of the reaction of phenylaalanine catalyzed by phenylalanine deaminase, which turns the slant green
Phenylalanine→PPA+NH3

Methyl Green?

Binds to DNA, when DNA is broken down by DNases, the blue color clears around the colony

Iodine

Is used in amylase test,

starch reacts with iodine to form a purple color, therefor a negative result has NO purple color

Spirit blue

Used for Lipase tests. Lipids and spirit blue associate in agar. If lipase is released by the colony, the lipid is broken down and spirit blue sticks to colony and the colony appears dull.

Endo agar

mEndo

mFc agar