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Sedimentation
Sedimentation
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12 Terms
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particle
A(n) ________ is accelerating till the coulombs force is equal to the frictional force.
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Isoelectric focusing
A) ________: separation of proteins based on isoelectric points (pH value where they have no net charge), it is the first step in 2D electrophoresis.
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sedimentation constant
The ________ gives the moving velocity of a particle at certain centrifugal acceleration.
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Centrifugal force
inertial force in a rotating system, it is radial from the center and equal to centripetal force
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Buoyancy
depends on volume immersed in liquid, density of the liquid and acceleration
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Drag force
resistance force caused by movement of a body (transferring energy to molecules) through a fluid
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a. Preparative
separation of molecules based on size and mass of molecules
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b. Analytical
determination of size and mass of particles
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Agarose gel
separating nucleic acid based on charge
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SDS PAGE
separation of protein based on its size using a vertical tank
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a) Isoelectric focusing
separation of proteins based on isoelectric points(pH value where they have no net charge), it is the first step in 2D electrophoresis
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b) 2D electrophoresis
the proteins are then separated based on their molecular weight by SDS-PAGE
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