Sedimentation

particle

particle

A(n) ________ is accelerating till the coulombs force is equal to the frictional force.

Isoelectric focusing

A) ________: separation of proteins based on isoelectric points (pH value where they have no net charge), it is the first step in 2D electrophoresis.

sedimentation constant

The ________ gives the moving velocity of a particle at certain centrifugal acceleration.

Centrifugal force

inertial force in a rotating system, it is radial from the center and equal to centripetal force

Buoyancy

depends on volume immersed in liquid, density of the liquid and acceleration

Drag force

resistance force caused by movement of a body (transferring energy to molecules) through a fluid

a. Preparative

separation of molecules based on size and mass of molecules

b. Analytical

determination of size and mass of particles

Agarose gel

separating nucleic acid based on charge

SDS PAGE

separation of protein based on its size using a vertical tank

a) Isoelectric focusing

separation of proteins based on isoelectric points(pH value where they have no net charge), it is the first step in 2D electrophoresis

b) 2D electrophoresis

the proteins are then separated based on their molecular weight by SDS-PAGE