Anatomy & Physiology I Lab (Chapter two: Microscope) Practical I

compound light microscope

What are microscopes used in lab called?

objective lens

Name the starred region in the image.

depth of field

____________ __________ ____________: How much of the total thickness of a specimen is in focus at each magnification.

mechanical stage

______________ __________: Platform that holds the slide in place.

stage aperture

____________ _______________: Opening in the stage that allows light to pass through the slide. Not shown in microscope images.

coarse adjustment knob

__________ ____________ ____________: Moves the stage up and down in large increments. NEVER use this knob when the high power objective lens is in place.

both

Transport: Always use ___________ hands while carrying the microscope.

lens paper

Lens care: Never use facial tissue, paper towels, clothing, water, or saliva to clean the micro scope lens. Ask for _________ ___________ to properly clean the lens.

scanning

Storage of microscope:

-Remove slide

-Rotate to the _____________ objective lens

-Lower stage

-Place back into cabinet

edges

Handling slides: Hold slides by the _____________ so you do not leave smudges. Only work with ONE slide at a time.

arm

Name the starred region in the image.

head

Name the starred region in the image.

base

Name the starred region in the image.

eyepieces

Name the starred region in the image.

ocular lenses

Use the starred image:

____________ ______________: Lenses located inside the eyepieces.

rotating nosepiece

Name the starred region in the image.

mechanical stage

Name the starred region in the image.

mechanical stage controls

Name the starred region in the image.

iris diaphragm

Name the starred region in the image.

coarse adjustment knob

Name the starred region in the image.

fine adjustment knob

Name the starred region in the image.

light source

Name the starred region in the image.

specimen slide

Name the starred region in the image.

stage

Name the starred region in the image.

10x

Ocular lens: magnifies ___________.

4x

Scanning objective lens: red ring; magnifies _________.

10x

Low power objective lens: yellow ring; magnifies _________.

40x

High power objective lens: blue ring; magnifies _________.

100x

Oil immersion: white ring; magnifies _________.

high power objective lens

Name the starred region in the image.

scanning objective lens

Name the starred region in the image.

low power objective lens

Name the starred region in the image.

total magnification

____________ _______________: Achieved through the interaction of two lenses: the ocular lens (10x) and the objective lens currently in use.

(ocular lens mag.) X (objective lens mag.) = _____________ _______________

40x

What is the total magnification when the scanning objective lens is in use?

100x

What is the total magnification when the low power objective lens is in use?

400x

What is the total magnification when the high power objective lens is in use?

1,000x

What is the total magnification when the oil immersion lens is in use?

parfocal

______________: If a slide is in clear focus on one objective, when you move to another objective whatever you are viewing will be in partial (almost clear) focus.

field of view

__________ ________ ____________: Everything that is visible when you look through the eyepiece.

working distance

____________ ______________: Distance between bottom of the objective lens to the top of the slide.

fine_

Only use the ___________ adjustment knob when on the high power objective lens.

arm

__________: Attaches the head, rotating nosepiece, and base together.

base

_____________: the bottom surface of the microscope.

head

___________: Part of the microscope that provides attachment points for the ocular and objective lenses.

eyepieces

_____________: Part of the microscope that you look into.

ocular lenses

____________ ___________: Lenses located inside the eyepieces. They magnify an object on the slider 10x its normal size.

rotating nosepiece

_____________ ______________: Connects the objective lenses to the head and rotates to change from one objective lens to another.

mechanical stage controls

______________ __________ ____________: Knobs used to move the stage left and right or up and down.

condenser

_______________: Located under the stage, used to focus light through the slide.

iris diaphragm

_________ _____________: Part of the condenser that opens and closes to allow different amounts of light through the condenser.

fine adjustment knob

__________ __________ ___________: Moves the stage up and down in small increments. ONLY use this knob when the high power objective lens is in place.

scanning objective lens

Which objective lens has the greatest field of view and the deepest depth of field.

oil immersion

_____________ _____________ objective lens: Largest objective lens. Magnifies 100x with a white band. ONLY used with a drop of oil on the cover slip.