Staining Technique Micro Lab Final

Slide Smear

Explain Slide Smear Technique

Slide smear technique is spreading a sample thinly on a slide for microscope viewing.

Heat fixing

Explain Heat Fixing Technique

Heat fixing is using heat to stick cells to a slide and preserve the structure while preventing contamination

Staining Method

Explain Staining Method Technique

Staining method is adding dye to a sample to color and highlight its parts for easier viewing under a microscope

Simple stain

Explain Simple Stain Technique

Simple stain is using one dye to color cells so their shape and arrangement are easier to see under a microscope (Colors cells or the background)

Direct Stain

Explain Direct Staining

Direct stain is a simple stain method that colors the cells or tissues themselves, leaving the background unstained or colorless

Negative Stain (Indirect)

Explain Negative Stain (Indirect)

Negative Stain is a simple stain method a method of preparing samples for electron microscopy where the background is stained, making the sample appear lighter in contrast

Differential Stain

Explain Differential Stain Technique

A process that uses multiple stains to distinguish between different types of microorganisms or structures/cellular components

Structural Stain

Explain Structural Stain Technique

helps in the visualization of a bacterial structure

Spore Stain

Explain Spore Stain Technique

A type of microbial staining technique used to identify and visualize bacterial endospores

Capsule Stain

Explain Capsule Stain Technique

A microscopic technique used to visualize bacterial capsules, which are protective, sticky, and often polysaccharide-rich layers surrounding some bacterial cells.

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Gram- Stain

Explain Gram Stain Technique

A staining technique for the preliminary identification of bacteria, in which a violet dye is applied, followed by a decolorizing agent and then a red dye. The cell walls of certain bacteria (denoted Gram-positive ) retain the first dye and appear violet, while those that lose it (denoted Gram-negative ) appear red. Also called Gram's method.

What color would be a gram positive?

Purple/Violet

What color would be a gram negative?

Red/Pink

What are the steps to Gram Stain Technique?

The steps include applying crystal violet stain, followed by iodine treatment, decolorization with alcohol or acetone, and finally counterstaining with safranin.

What kind of stain is Gram Stain?

Differential

What kind of stain is Acid-Fast Stain?

Differential

Acid-Fast Stain

Which stain is better to use Basic or acidic stain?

Basic stain because generally all the cell have a negative charge on the outside of their cell wall and basic stain work with the negative stain and allow the cell to stick and colorize JUST the cell. (the acidic stain makes the cell clear)

Difference between Direct and Negative (indirect) stain?

Direct uses heat fixing and Negative stain does not use heat fixing

Fixation

staining method that keeps bacteria preserved on the slide and kills bacteria

chemical fixing

uses chemicals to preserve the structure of the sample

Also:

-stops cell degradation

- denatures proteins and stops enzymatic activity within specimen

chromophore stands for..

Chromo: color

Phore: bearing

basic stain

direct stain/positively charged chromophore (color bearing)

acidic stain

indirect stain/negatively charge chromophore