Histo Sem W2: Fixation

room temperature

traditional temperature

heated at 60C

urgent biopsies temperature

0-4C

electron microscopy temperature

room temperature

mast cells temperature

100C

formalin temperature

formalin at 100C

can be used to fix tissues with TB

45C

formalin with RNA

65C

formalin with DNA

2cm²

cross section thickness

<4mm

thickness general

isotonic

osmolality dapat for fixatives

slightly hypertonic solutions

best results osmolality are usually obtained at

10%

formalin concentration

3%

routine Glutaraldehyde concentration

0.25%

electron microscopy Glutaraldehyde concentration

1:20

volume (tissue:fixative)

4-6 hours

duration - adequate fixation

shrinkage, hardening, and may inhibit chemical reactions

prolonged fixation

thin tissue sections

hasten

agitation

hasten

moderate heat

hasten

thick tissue sections

retarded

presence of mucus

retarded

presence of fats

retarded

presence of blood

retarded

cold temperature

retarded