Cells

What are the distinguishing features of eukaryotic cells?

What are the distinguishing features of eukaryotic cells?

● Cytoplasm containing membrane-bound organelles

● So DNA enclosed in a nucleus

Describe the general structure of eukaryotic cells

Describe the structure of the cell-surface membrane

Describe the function of the cell-surface membrane

● Selectively permeable → enables control of passage of substances in / out of cell

● Molecules / receptors / antigens on surface → allow cell recognition / signalling

Describe the structure of the nucleus

Describe the function of the nucleus

● Holds / stores genetic information which codes for polypeptides (proteins)

● Site of DNA replication

● Site of transcription (part of protein synthesis), producing mRNA

● Nucleolus makes ribosomes / rRNA

Describe the structure of a ribosome

● Made of ribosomal RNA and protein (two subunits)

● Not a membrane-bound organelle

Describe the function of a ribosome

Site of protein synthesis (translation)

Describe the structure of rough (rER) & smooth endoplasmic reticulum (sER)

Describe the function of rER and sER

Describe the structure of Golgi apparatus and Golgi vesicles

Describe the function of Golgi apparatus and Golgi vesicles

Describe the structure of lysosomes

Describe the function of lysosomes

● Release hydrolytic enzymes (lysozymes)

● To break down / hydrolyse pathogens or worn-out cell components

Describe the structure of mitochondria

Describe the function of mitochondria

● Site of aerobic respiration

● To produce ATP for energy release

● Eg. for protein synthesis / vesicle movement / active transport

Describe the structure of chloroplasts in plants and algae

Describe the function of chloroplasts in plants and algae

● Absorbs light energy for photosynthesis

● To produce organic substances eg. carbohydrates / lipid

Describe the structure of the cell wall in plants, algae and fungi

● Composed mainly of cellulose (polysaccharide) in plants / algae

● Composed of chitin (a nitrogen-containing polysaccharide) in fungi

Describe the function of the cell wall in plants, algae and fungi

● Provides mechanical strength to cell

● So prevents cell changing shape or bursting under pressure due to osmos

Describe the structure of the cell vacuole in plants

Describe the function of the cell vacuole in plants

● Maintains turgor pressure in cell (stopping plant wilting)

● Contains cell sap → stores sugars, amino acids, pigments and any waste chemicals

Describe how eukaryotic cells are organised in complex multicellular organisms

Describe how you can apply your knowledge of cell features / organelles to explain adaptations of eukaryotic cells

● [Named cell] has many [named organelle, eg. ribosomes]

● To [link organelle function to cell function eg. increase rate of protein synthesis, making many antibodies]

Exam insight: common mistakes

What are the distinguishing features of prokaryotic cells?

● Cytoplasm lacking membrane-bound organelles

● So genetic material not enclosed in a nucleus

Describe the general structure of prokaryotic cells

Compare and contrast the structure of eukaryotic and prokaryotic cells

Describe the general structure of a virus particle

1. Nucleic acids surrounded by a capsid (protein coat)

2. Attachment proteins allow attachment to specific host cells

3. No cytoplasm, ribosomes, cell wall, cell-surface membrane etc.

4. Some also surrounded by a lipid envelope eg. HIV

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Describe the difference between magnification and resolution

● Magnification = number of times greater image is than size of the real (actual) object

○ Magnification = size of image / size of real object

● Resolution = minimum distance apart 2 objects can be to be distinguished as separate objects

Compare the principles and limitations of optical microscopes, transmission electron microscopes and scanning electron microscopes

Students should be able to appreciate that there was a considerable period of time during which the scientific community distinguished between artefacts (eg. dust, air bubbles occurring during preparation) and cell organelles.

To overcome this, scientists prepared specimens in different ways. If an object was seen with one technique but not another, it was more likely to be an artefact than an organelle.

List the steps in calculations involving magnification, real size & image size

Describe how to convert between different units

Describe how the size of an object viewed with an optical microscope can be measured

Eg. using the stage micrometre to calculate the size of divisions on the eyepiece graticule.

1. Line up (scale of) eyepiece graticule with (scale of) stage micrometre

2. Calibrate eyepiece graticule - use stage micrometre to calculate size of divisions on eyepiece graticule

3. Take micrometre away and use graticule to measure how many divisions make up the object

4. Calculate size of object by multiplying number of divisions by size of division

5. Recalibrate eyepiece graticule at different magnifications

● 4 eyepiece graticule divisions = 10 stage micrometre divisions

● In this stage micrometre, 1 subdivision = 10 μm

● So 4 eyepiece graticule divisions = 10μmx10=100μm

● So 1 eyepiece graticule division = 100 μm/4 = 25 μm

Describe and explain the principles of cell fractionation and ultracentrifugation as used to separate cell components

1. Homogenise tissue / use a blender

● Disrupts cell membrane, breaking open cells and releasing contents / organelles

2. Place in a cold, isotonic, buffered solution

● Cold to reduce enzyme activity → so organelles not broken down / damaged

● Isotonic so water doesn’t move in or out of organelles by osmosis → so they don’t burst

● Buffered to keep pH constant → so enzymes don’t denature

3. Filter homogenate

● Remove large, unwanted debris eg. whole cells, connective tissue

4. Ultracentrifugation - separates organelles in order of density / mass

● Centrifuge homogenate in a tube at a high speed

● Remove pellet of heaviest organelle and respin supernatant at a higher speed

● Repeat at increasing speeds until separated out, each time pellet made of lighter

organelles (nuclei → chloroplasts / mitochondria → lysosomes → ER → ribosomes)

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Describe the stages of the cell cycle in eukaryotic cells

Describe the behaviour of chromosomes & role of spindle fibres in mitosis

Students should be able to recognise the stages of the cell cycle and explain the appearance of cells in each stage of mitosis.

This is covered under required practical

Why do some eukaryotic cells not undergo the cell cycle?

● Within multicellular organisms, not all cells retain the ability to divide (eg. neurons)

● Only cells that do retain this ability go through a cell cycle

Explain the importance of mitosis in the life of an organism

Parent cell divides to produce 2 genetically identical daughter cells for...

• Growth of multicellular organisms by increasing cell number

• Replacing cells to repair damaged tissues

• Asexual reproduction

Describe how tumours and cancers form

Mitosis is a controlled process.

● Mutations in DNA / genes controlling mitosis can lead to uncontrolled cell division

● Tumour formed if this results in mass of abnormal cells

○ Malignant tumour = cancerous, can spread (metastasis)

○ Benign tumour = non-cancerous

Suggest how cancer treatments control rate of cell division

● Some disrupt spindle fibre activity / formation

○ So chromosomes can’t attach to spindle by their centromere

○ So chromatids can’t be separated to opposite poles (no anaphase)

○ So prevents / slows mitosis

● Some prevent DNA replication during interphase

○ So can’t make 2 copies of each chromosome (chromatids)

○ So prevents / slows mitosis

Describe how prokaryotic cells replicate

Binary Fission:

1. Replication of circular DNA

2. Replication of plasmids

3. Division of cytoplasm to produce 2 daughter cells

● Single copy of circular DNA

● Variable number of copies of plasmids

Describe how viruses replicate

Being non-living, viruses do not undergo cell division.

1. Attachment proteins attach to complementary receptors on host cell

2. Inject viral nucleic acid (DNA/RNA) into host cell

3. Infected host cell replicates virus particles:

a. Nucleic acid replicated

b. Cell produces viral protein / capsid / enzymes

c. Virus assembled then released

Exam insight: common mistakes

Describe the fluid-mosaic model of membrane structure

The basic structure of all cell membranes (cell-surface membranes & membranes around eukaryotic organelles) is the same.

● Molecules free to move laterally in phospholipid bilayer

● Many components - phospholipids, proteins, glycoproteins and glycolipids

Describe the arrangement of the components of a cell membrane

● Phospholipids form a bilayer - fatty acid tails face inwards, phosphate heads face outwards

● Proteins

○ Intrinsic / integral proteins span bilayer eg. channel and carrier proteins

○ Extrinsic / peripheral proteins on surface of membrane

● Glycolipids (lipids with polysaccharide chains attached) found on exterior surface

● Glycoproteins (proteins with polysaccharide chains attached) found on exterior surface

● Cholesterol (sometimes present) bonds to phospholipid hydrophobic fatty acid tails

Explain the arrangement of phospholipids in a cell membrane

• Bilayer, with water present on either side

• Hydrophobic fatty acid tails repelled from water so point away from water / to interior

• Hydrophilic phosphate heads attracted to water so point to water

Explain the role of cholesterol (sometimes present) in cell membranes

● Restricts movement of other molecules making up membrane

● So decreases fluidity (and permeability) / increases rigidity

Suggest how cell membranes are adapted for other functions

● Phospholipid bilayer is fluid → membrane can bend for vesicle formation / phagocytosis

● Glycoproteins / glycolipids act as receptors / antigens → involved in cell signalling / recognition

Explain the limitations imposed by the nature of the phospholipid bilayer

● Restricts movement of water soluble (polar) & larger substances eg. Na+ / glucose

● Due to hydrophobic fatty acid tails in interior of bilayer

Describe how movement across membranes occurs by facilitated diffusion

● Water-soluble (polar) / slightly larger substances

● Move down a concentration gradient

● Through specific channel / carrier proteins

● Passive - doesn’t require energy from ATP / respiration (only kinetic energy of substances)

Explain the role of carrier and channel proteins in facilitated diffusion

● Shape / charge of protein determines which substances move

● Channel proteins facilitate diffusion of water-soluble substances

○ Hydrophilic pore filled with water

○ May be gated - can open / close

● Carrier proteins facilitate diffusion of (slightly larger) substances

○ Complementary substance attaches to binding site

○ Protein changes shape to transport substance

Describe how movement across membranes occurs by osmosis

Water potential is a measure of how likely water molecules are to move out of a solution. Pure (distilled) water has the maximum possible ψ (0 kPA), increasing solute concentration decreases ψ.

● Water diffuses / moves

● From an area of high to low water potential (ψ) / down a water potential gradient

● Through a partially permeable membrane

● Passive - doesn’t require energy from ATP / respiration (only kinetic energy of substances)

Describe how movement across membranes occurs by active transport

● Substances move from area of lower to higher concentration / against a concentration gradient

● Requiring hydrolysis of ATP and specific carrier proteins

Describe the role of carrier proteins and the importance of the hydrolysis of ATP in active transport

1. Complementary substance binds to specific carrier protein

2. ATP binds, hydrolysed into ADP + Pi, releasing energy

3. Carrier protein changes shape, releasing substance on side of higher concentration

4. Pi released → protein returns to original shape

Describe how movement across membranes occurs by co-transport

● Two different substances bind to and move simultaneously via a co-transporter protein (type of carrier protein)

● Movement of one substance against its concentration gradient is often coupled with the movement of another down its concentration gradient

Describe an example that illustrates co-transport

Absorption of sodium ions and glucose (or amino acids) by cells lining the mammalian ileum:

● Na+ actively transported from epithelial cells to blood (by Na+/K+ pump)

● Establishing a conc. gradient of Na+ (higher in lumen than epithelial cell)

● Na+ enters epithelial cell down its concentration gradient with glucose against its concentration gradient

● Via a co-transporter protein

● Glucose moves down a conc. gradient into blood via facilitated diffusion

The movement of sodium can be considered indirect / secondary active transport, as it is reliant on a concentration gradient established by active transport.

Describe how surface area, number of channel or carrier proteins and differences in gradients of concentration or water potential affect the rate of movement across cell membranes

● Increasing surface area of membrane increases rate of movement

● Increasing number of channel / carrier proteins increases rate of facilitated diffusion / active transport

● Increasing concentration gradient increases rate of simple / facilitated diffusion and osmosis

● Increasing concentration gradient increases rate of facilitated diffusion

○ Until number of channel / carrier proteins becomes a limiting factor as all in use / saturated ● Increasing water potential gradient increases rate of osmosis

Explain the adaptations of some specialised cells in relation to the rate of transport across their internal and external membranes

● Membrane folded eg. microvilli in ileum → increase in surface area

● More protein channels / carriers → for facilitated diffusion (or active transport - carrier proteins only)

● Large number of mitochondria → make more ATP by aerobic respiration for active transport

What is an antigen?

● Foreign molecule / protein / glycoprotein / glycolipid

● That stimulates an immune response leading to production of antibody

How are cells identified by the immune system?

● Each type of cell has specific molecules on its surface (cell-surface membrane / cell wall) that identify it

● Often proteins → have a specific tertiary structure (or glycoproteins / glycolipids)

What types of cells and molecules can the immune system identify?

1. Pathogens (disease causing microorganisms) eg. viruses, fungi, bacteria

2. Cells from other organisms of the same species (eg. organ transplants)

3. Abnormal body cells eg. tumour cells or virus-infected cells

4. Toxins (poisons) released by some bacteria

Describe phagocytosis of pathogens (non-specific immune response)

• Phagocyte attracted by chemicals / recognises (foreign) antigens on pathogen

• Phagocyte engulfs pathogen by surrounding it with its cell membrane

• Pathogen contained in vesicle / phagosome in cytoplasm of phagocyte

• Lysosome fuses with phagosome and releases lysozymes (hydrolytic enzymes)

• Lysozymes hydrolyse / digest pathogen

What does phagocytosis lead to?

• Phagocytosis leads to presentation of antigens where antigens are displayed on the phagocyte cell-surface membrane, stimulating the specific immune response (cellular and humoral response).

Describe the response of T lymphocytes to a foreign antigen (the cellular response)

T lymphocytes recognise (antigens on surface of) antigen presenting cells eg. infected cells, phagocytes presenting antigens, transplanted cells, tumour cells etc.

Specific helper T cells with complementary receptors (on cell surface) bind to antigen on antigen-presenting cell → activated and divide by mitosis to form clones which stimulate:

● Cytotoxic T cells → kill infected cells / tumour cells (by producing perforin)

● Specific B cells (humoral response - see below)

● Phagocytes → engulf pathogens by phagocytosis

Describe the response of B lymphocytes to a foreign antigen (the humoral response)

B lymphocytes can recognise free antigens eg. in blood or tissues, not just antigen presenting cells.

1. Clonal selection:

● Specific B lymphocyte with complementary receptor (antibody on cell surface) binds to antigen ● This is then stimulated by helper T cells (which releases cytokines)

● So divides (rapidly) by mitosis to form clones

2. Some differentiate into B plasma cells → secrete large amounts of (monoclonal) antibody

3. Some differentiate into B memory cells → remain in blood for secondary immune response

What are antibodies?

● Quaternary structure proteins (4 polypeptide chains)

● Secreted by B lymphocytes eg. plasma cells in response to specific antigens

● Bind specifically to antigens forming antigen-antibody complexes

Describe the structure of an antibody

Explain how antibodies lead to the destruction of pathogens

● Antibodies bind to antigens on pathogens forming an antigen-antibody complex

○ Specific tertiary structure so binding site / variable region binds to complementary antigen

● Each antibody binds to 2 pathogens at a time causing agglutination (clumping) of pathogens

● Antibodies attract phagocytes

● Phagocytes bind to the antibodies and phagocytose many pathogens at once

What is a vaccine?

● Injection of antigens from attenuated (dead or weakened) pathogens

● Stimulating formation of memory cells

Explain how vaccines provide protection to individuals against disease

1. Specific B lymphocyte with complementary receptor binds to antigen

2. Specific T helper cell binds to antigen-presenting cell and stimulates B cell

3. B lymphocyte divides by mitosis to form clones

4. Some differentiate into B plasma cells which release antibodies

5. Some differentiate into B memory cells

6. On secondary exposure to antigen, B memory cells rapidly divide by mitosis to produce B plasma cells

7. These release antibodies faster and at a higher concentration

Explain how vaccines provide protections for populations against disease

● Herd immunity - large proportion of population vaccinated, reducing spread of pathogen

○ Large proportion of population immune so do not become ill from infection

○ Fewer infected people to pass pathogen on / unvaccinated people less likely to come in contact with someone with disease

Describe the differences between active and passive immunity

Explain the effect of antigen variability on disease and disease prevention

● Antigens on pathogens change shape / tertiary structure due to gene mutations (creating new strains)

● So no longer immune (from vaccine or prior infection)

○ B memory cell receptors cannot bind to / recognise changed antigen on secondary exposure

○ Specific antibodies not complementary / cannot bind to changed antigen

Describe the structure of a HIV particle

Describe the replication of HIV in helper T cells

1. HIV attachment proteins attach to receptors on helper T cell

2. Lipid envelope fuses with cell-surface membrane, releasing capsid into cell

3. Capsid uncoats, releasing RNA and reverse transcriptase

4. Reverse transcriptase converts viral RNA to DNA

5. Viral DNA inserted / incorporated into helper T cell DNA (may remain latent)

6. Viral protein / capsid / enzymes are produced

a. DNA transcribed into HIV mRNA

b. HIV mRNA translated into new HIV proteins

7. Virus particles assembled and released from cell (via budding)

Explain how HIV causes the symptoms of acquired immune deficiency syndrome (AIDS)

● HIV infects and kills helper T cells (host cell) as it multiplies rapidly

○ So T helper cells can’t stimulate cytotoxic T cells, B cells and phagocytes

○ So B plasma cells can’t release as many antibodies for agglutination & destruction of pathogens

● Immune system deteriorates → more susceptible to (opportunistic) infections

● Pathogens reproduce, release toxins and damage cells

Explain why antibiotics are ineffective against viruses

Viruses do not have structures / processes that antibiotics inhibit: ● Viruses do not have metabolic processes (eg. do not make protein) / ribosomes

● Viruses do not have bacterial enzymes / murein cell wall

What is a monoclonal antibody?

● Antibody produced from genetically identical / cloned B lymphocytes / plasma cells

● So have same tertiary structure

Explain how monoclonal antibodies can be used in medical treatments

● Monoclonal antibody has a specific tertiary structure / binding site / variable region

● Complementary to receptor / protein / antigen found only on a specific cell type (eg. cancer cell)

● Therapeutic drug attached to antibody

● Antibody binds to specific cell, forming antigen-antibody complex, delivering drug

Explain how monoclonal antibodies can be used in medical diagnosis

● Monoclonal antibody has a specific tertiary structure / binding site / variable region

● Complementary to specific receptor / protein / antigen associated with diagnosis

● Dye / stain / fluorescent marker attached to antibody

● Antibody binds to receptor / protein / antigen, forming antigen-antibody complex

Explain the use of antibodies in the ELISA (enzyme-linked immunosorbent assay) test to detect antigens

Direct ELISA

1. Attach sample with potential antigens to well

2. Add complementary monoclonal antibodies with enzymes attached → bind to antigens if present

3. Wash well → remove unbound antibodies (to prevent false positive)

4. Add substrate → enzymes create products that cause a colour change (positive result)

OR sandwich ELISA

1. Attach specific monoclonal antibodies to well

2. Add sample with potential antigens, then wash well

3. Add complementary monoclonal antibodies with enzymes attached → bind to antigens if present

4. Wash well → remove unbound antibodies (to prevent false positive)

5. Add substrate → enzymes create products that cause a colour change (positive result)

Explain the use of antibodies in the ELISA test to detect antibodies

Indirect ELISA:

1. Attach specific antigens to well

2. Add sample with potential antibodies, wash well

3. Add complementary monoclonal antibodies

with enzymes attached → bind to antibodies if present

4. Wash well → remove unbound antibodies

5. Add substrate → enzymes create products that cause a colour change (positive result)

Suggest the purpose of a control well in the ELISA test

● Compare to test to show only enzyme causes colour change

● Compare to test to show all unbound antibodies have been washed away

Discuss some general ethical issues associated with the use of vaccines and monoclonal antibodies

● Pre-clinical testing on / use of animals - potential stress / harm / mistreatment

○ But animals not killed & helps produce new drugs to reduce human suffering

● Clinical trials on humans - potential harm / side-effects

● Vaccines - may continue high risk activities and still develop / pass on pathogen

● Use of drug - potentially dangerous side effects

Suggest some points to consider when evaluating methodology relating to the use of vaccines and monoclonal antibodies

● Was the sample size large enough to be representative?

● Were participants diverse in terms of age, sex, ethnicity and health status?

● Were placebo / control groups used for comparison?

● Was the duration of the study long enough to show long-term effects?

● Was the trial double-blind (neither doctor / patient knew who was given drug or placebo) to reduce bias?

Suggest some points to consider when evaluating evidence and data relating to the use of vaccines and monoclonal antibodies

● What side effects were observed, and how frequently did they occur?

● Was a statistical test used to see if there was a significant difference between start & final results?

● Was the standard deviation of final results large, showing some people did not benefit?

● Did standard deviations of start & final results overlap, showing there may not be a significant difference?

● What dosage was optimum? Does increasing dose increase effectiveness enough to justify extra cost?

● Was the cost of production & distribution low enough?

Exam insight: common mistakes