CC2_Prelim_Lab: ACP Determination

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Last updated 4:35 PM on 9/20/26
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13 Terms

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  1. 1mL of ACP Working rgt

  2. Add 100uL of stabilized Serum sample

  3. incubate for 5 mins at 37 C

  4. Read and recorde Absorbance for every minute for 5 mimutes (5 Abs. reading) with a wavelength of 405 nm

  5. Calculate Abs/min


Procedure for Total ACP

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  1. 1mL Working rgt

  2. 10 uL of L-Tartrate

  3. 100 uL of stabilized Serum sample

  4. incubate for 5 mins at 37 C

  5. Get Abs every minute for 5 mins. (5 Abs readings)

  6. Calculate for Abs/min



Procedure for nonp-ACP

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  • 24 hrs a@ RT

  • 14 days @ 2-8 C (Ref temp)


Rgt stability of ACP working rgt.

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ACP Working rgt. Abs > 300

What abs to reject ACP Working rgt

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  • Serum

  • separate from clot within 2 hrs. after collection


Accepted specimen for ACP

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7 days at 2-8 C

Stabilized serum Stability?

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2.5-11.7 U/L at 37 C

Ref value for Total ACP

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0.2-3.5 U/L at 37 C

Ref value for pACP

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Total ACP - nonp-ACP

Formula for pACP

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40 U/L

Linearity of the protocol for ACP

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Hydrolyze Phosphate at Acidic pH

Role of Acid Phosphatase

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Diagnosis of disorders related to the prostate

ex. Prostatic carcinoma

Clinical Significance of ACP

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RBC, WBC, Plt, and Bones

Nonp-ACP can be found in