MICRO LAB [Inoculation, Morphology, Hemolysis]

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43 Terms

1
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Streak plate method

What set-up?

<p>What set-up?</p>
2
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To isolate single colonies /
To isolate the organism in pure culture

What is the rationale or purpose for this set-up?

<p>What is the rationale or purpose for this set-up?</p>
3
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Streak plate method

What set-up?

<p>What set-up?</p>
4
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4th quadrant

Which quadrant should have isolated colonies?

<p>Which quadrant should have isolated colonies?</p>
5
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Punctiform (tiny)

Identify the SHAPE

<p>Identify the <strong>SHAPE</strong></p>
6
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Circular

Identify the SHAPE

<p>Identify the <strong>SHAPE</strong></p>
7
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Irregular

Identify the SHAPE

<p>Identify the <strong>SHAPE</strong></p>
8
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Entire (smooth)

Identify the MARGIN

<p>Identify the <strong>MARGIN</strong></p>
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Undulate (wavy)

Identify the MARGIN

<p>Identify the <strong>MARGIN</strong></p>
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Rhizoid

Identify the MARGIN

<p>Identify the <strong>MARGIN</strong></p>
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Lobate

Identify the MARGIN

<p>Identify the <strong>MARGIN</strong></p>
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Filamentous

Identify the MARGIN

<p>Identify the <strong>MARGIN</strong></p>
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Flat

Identify the ELEVATION

<p>Identify the <strong>ELEVATION</strong></p>
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Convex

Identify the ELEVATION

<p>Identify the <strong>ELEVATION</strong></p>
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Umbonate

Identify the ELEVATION

<p>Identify the <strong>ELEVATION</strong></p>
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Raised

Identify the ELEVATION

<p>Identify the <strong>ELEVATION</strong></p>
17
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Turbidity

The cloudy appearance of a liquid medium due to the presence of bacteria

18
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A = None
B = Light
C = Moderate
D = Heavy

Identify the TURBIDITY

<p>Identify the TURBIDITY</p>
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A = 0-16^6
B = 10^7
C = 10^8
D = 10^9

Identify the estimated # Bacteria per mL

<p>Identify the estimated # Bacteria per mL</p>
20
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3 x 10^9

Usually bacterial populations do not exceed ________ bacteria/mL when grown in liquid media.

21
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Blood Agar Plate

a rich, complex medium that contains 5% sheep red blood cells

22
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Blood Agar Plate

What plate?

<p>What plate?</p>
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Blood Agar Plate

- tests the ability of an organism to produce hemolysins, enzymes that damage/lyse red blood cells (erythrocytes).

24
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Beta-hemolysis

_________ is complete hemolysis

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Beta-hemolysis

It is characterized by a clear (transparent) zone surrounding the colonies

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Beta-hemolysis

What type of Hemolysis?

<p>What type of Hemolysis?</p>
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Beta-hemolysis

Staphylococcus aureus

Streptococcus pyogenes

Streptococcus agalactiae

What type of Hemolysis? Give example of organism w/ this type

<p>What type of <strong>Hemolysis</strong>? Give example of organism w/ this type</p>
28
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Alpha-hemolysis

Partial hemolysis is termed _________

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Alpha-hemolysis

Colonies typically are surrounded by a green, opaque zone

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Alpha-hemolysis

What type of hemolysis?

<p>What type of <strong>hemolysis</strong>?</p>
31
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Alpha-hemolysis

Streptococcus pneumoniae

Streptococcus mitis

What type of hemolysis?

Give example of organism

<p>What type of <strong>hemolysis</strong>? </p><p></p><p>Give example of organism</p>
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Gamma-hemolysis

What type of hemolysis?

<p>What type of <strong>hemolysis</strong>?</p>
33
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Gamma-hemolysis

Staphylococcus epidermidis

What type of hemolysis?

Give example

<p>What type of <strong>hemolysis</strong>? </p><p></p><p>Give example</p>
34
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Gamma-hemolysis

- no hemolysis
- no notable zones around the colonies

35
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Streak-stab technique

Often when inoculating a BAP to observe hemoloysis patterns, investigators will also stab several times through the agar using an inoculating loop. What do you call this technique?

36
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streptolysin O

This stab allows for the detection of ___________, a specific hemolysin produced by Streptococcus pyogenes.

<p>This stab allows for the detection of ___________, a specific <strong>hemolysin</strong> produced by <strong><em>Streptococcus pyogenes</em>.</strong></p>
37
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Streak-stab technique

What technique is this?

<p>What technique is this?</p>
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streptolysin O

What is detected in this technique?

Note the oval-shaped areas of clearing around the stab marks in the picture; these are caused by __________

<p>What is detected in this technique? </p><p></p><p>Note the <strong>oval-shaped areas</strong> of clearing around the stab marks in the picture; these are caused by __________</p>
39
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Streptococcus pyogenes

streptolysin O is a specific hemolysin produced by ___________

40
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Aseptic technique

What do you call this technique?

<p>What do you call this technique?</p>
41
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Aseptic technique

For sterilization

What do you call this technique?

What is it's purpose?

<p>What do you call this technique?<br><br>What is it's purpose?</p>
42
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Broth culturing

What type of culture?

<p>What type of culture?</p>
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35-37C @ 24-48hours

How many minutes/hours and at what temperature is the incubation of culture media?

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